We narrowed to 565 results for: mme

1. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   ...the PAM sequence. Avoid targeting a ‘C’ placed immediately after a ‘G’ base because CBE is inefficient in...

2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   ...corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death." Proceedings of the National...

3. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   ...people on twitter and maintain a connection by commenting and sharing. LinkedIn or ResearchGate allow you...

4. Twenty Years of Sharing: Expanding Accessibility

   Type

   Blog Post

   ... media, on collaborators’ websites, or as a recommendation in a Reddit thread crowdsourcing experimental...

5. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   ...Suggest a viral vector for our service; leave a comment; or take our AAV survey. Learn more about GCaMPs...

6. Antibodies 101: The Four ELISAs and When to Use Them

   Type

   Blog Post

   ...Users may also find it difficult to source a commercially available and appropriately conjugated antibody...

7. Hot Plasmids Spring 2024

   Type

   Blog Post

   ...component for viral genome packaging. Addgene recommends both for use in western blots (Figure 3).    ...

8. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ...an academic lab to industry without being too commercial or “sales-y”. The work includes reviewing customer...

9. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   ...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...

10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    ... antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest project to develop...

11. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    ...performance. By this point, towards the end of the summer, things were getting better and the office had ...

12. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    ...tolerate a mismatch in the binding sequence, we recommend changing several bases as close to the PAM as ...

13. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    ... cut vector with excellent efficiency using a commercial isothermal assembly master mix. Introduction ...

14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    ...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells." Cell 143.1 (2010)...

15. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    ...tagging vectors for your specific gene, the lab recommends designing homology arms with the modification...

16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    ...10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based ...

17. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    ...should validate it for your particular setup. We recommend starting by searching the literature to see what...

18. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    ...with all plasmids in the repository, we highly recommend reading the associated publications or protocols...

19. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    ... have any questions, please write to us in the comments section.  FX cloning kit This kit is designed...

20. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    ...extensively worked with and optimized Sep-OTSλ and can recommend its use (6).  2. C321, a specialized bacterial...