We narrowed to 560 results for: mme

1. Antibodies 101: The Four ELISAs and When to Use Them

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   Blog Post

   ...Users may also find it difficult to source a commercially available and appropriately conjugated antibody...

2. Hot Plasmids Spring 2024

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   ...component for viral genome packaging. Addgene recommends both for use in western blots (Figure 3).    ...

3. Transferable Skills Guide: Creativity

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   ...person speaks up after a group consensus and is immediately followed by several other people with their own...

4. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...tagging vectors for your specific gene, the lab recommends designing homology arms with the modification...

5. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   ...tolerate a mismatch in the binding sequence, we recommend changing several bases as close to the PAM as ...

6. Harnessing Bacterial Toxins for Allelic Exchange

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   ... cut vector with excellent efficiency using a commercial isothermal assembly master mix. Introduction ...

7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells." Cell 143.1 (2010)...

8. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based ...

9. Transferable Skills Guide: Conflict Resolution

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   ...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...

10. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ... antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest project to develop...

11. Filming Science Videos in the Age of Social Distancing

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    ...performance. By this point, towards the end of the summer, things were getting better and the office had ...

12. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...should validate it for your particular setup. We recommend starting by searching the literature to see what...

13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...with all plasmids in the repository, we highly recommend reading the associated publications or protocols...

14. Multiple Plasmids at a Low Price: Kits from Addgene

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    ... have any questions, please write to us in the comments section.  FX cloning kit This kit is designed...

15. Using Phosphoserine to Study Protein Phosphorylation

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    ...extensively worked with and optimized Sep-OTSλ and can recommend its use (6).  2. C321, a specialized bacterial...

16. Thoughts on the Future of Research 2015 Boston Symposium

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    ...develop a plan to make postdoctoral pay/benefits commensurate with work training and experience, better align...

17. How to Negotiate a Successful Lab Start Up Budget

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    ... negotiating budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz...

18. Viral Vectors 101: Optogenetic Tools

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    ...help light your path! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic...

19. Plasmids 101: Repressible Promoters

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    Blog Post

    ..., the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6...

20. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...