We narrowed to 568 results for: mme

1. Twenty Years of Sharing: Expanding Accessibility

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   Blog Post

   ... media, on collaborators’ websites, or as a recommendation in a Reddit thread crowdsourcing experimental...

2. Twenty Years of Sharing: Addgene's Viral Vector Service

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   Blog Post

   ...Suggest a viral vector for our service; leave a comment; or take our AAV survey. Learn more about GCaMPs...

3. Antibodies 101: The Four ELISAs and When to Use Them

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   Blog Post

   ...Users may also find it difficult to source a commercially available and appropriately conjugated antibody...

4. Hot Plasmids Spring 2024

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   Blog Post

   ...component for viral genome packaging. Addgene recommends both for use in western blots (Figure 3).    ...

5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ... non-specific cleavage is thought to activate programmed cell death or a dormant state for bacterial cells...

6. Transferable Skills Guide: Creativity

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   Blog Post

   ...person speaks up after a group consensus and is immediately followed by several other people with their own...

7. Career Insights: Technical Support Specialist

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   Blog Post

   ...an academic lab to industry without being too commercial or “sales-y”. The work includes reviewing customer...

8. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   Blog Post

   ...Gunn  and Dr. Denise M. Akob for reviewing and commenting. Binning Singletons is especially grateful to...

9. Lab to Office Culture Shock

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   Blog Post

   ...different. Some of these changes you may enjoy immediately and some of them may just take some getting used...

10. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...the PAM sequence. Avoid targeting a ‘C’ placed immediately after a ‘G’ base because CBE is inefficient in...

11. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...done largely in vitro; Easy and reproducible commercial synthesis (those of us who have used antibodies...

12. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

    ... cut vector with excellent efficiency using a commercial isothermal assembly master mix. Introduction ...

13. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells." Cell 143.1 (2010)...

14. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

    ...tolerate a mismatch in the binding sequence, we recommend changing several bases as close to the PAM as ...

15. Transferable Skills Guide: Conflict Resolution

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    Blog Post

    ...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...

16. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post

    ...should validate it for your particular setup. We recommend starting by searching the literature to see what...

17. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post

    ...10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based ...

18. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post

    ...tagging vectors for your specific gene, the lab recommends designing homology arms with the modification...

19. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post

    ... antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest project to develop...

20. Filming Science Videos in the Age of Social Distancing

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    Blog Post

    ...performance. By this point, towards the end of the summer, things were getting better and the office had ...