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  1. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... in a particular organism. Why is codon usage important? Translation and protein synthesis relies on transfer...(Mauro and Chappell 2014).  Ultimately, it is important to take a close look at your experiment and gene...

  2. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    ... passive mode, the stronger is the depressive component of the animal behavior. With the availability ...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

  4. Neuronal labeling with Spaghetti Monster

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    Blog Post
    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...fluorescent proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags. Indeed,...

  5. Tips for Using FRET in Your Experiments

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    Blog Post
    ... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

  6. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post
    ...Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation...21408-21413. PUF Assembly Kit PUF proteins are composed of repeat segments that can be engineered to bind...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...which could potentially truncate the template). Importantly, you also need a DNA polymerase that produces...resulting construct. In cases where the functionally important regions span several kb (or are GC-rich and therefore...

  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in...Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat...

  9. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post
    ...generations of scFvs against two antigens that are impossible to generate scFvs against in vivo: antigens found...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ...

  10. CRISPR 101: Homology Directed Repair

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    Blog Post
    ...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...

  11. Hot Biosensors 2022: Year-End Roundup

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    Blog Post
    ...plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling...sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a binding affinity...

  12. Hot Plasmids: Summer 2024

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    Blog Post
    ...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...Ashley Waldron The mammalian nervous system is composed of myriad cell types and subtypes, and a familiar...

  14. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...(FLuc) and Renilla (RLuc) luciferase. This is important not so much for how bright you can make a reaction...fusion protein called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca...

  15. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Serotypes Serotype refers to the different protein compositions of AAV capsids, which determine how a virus ...et al., 2014; Nieuwenhuis et al., 2021). It is important to note that this expression pattern can be affected...

  16. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

  17. CRISPR 101: Off-Target Effects

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    Blog Post
    ...unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand them...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...of the gene with multiple gRNAs, but the most important is that you could avoid potential off target artifacts...you could run another gel that highlights the important results (for example, if you needed to make a ...
Showing: 481 - 500 of 750 results