We narrowed to 759 results for: mpo

... example all C’s will edit to T’s. Thus, it’s important that there are no “bystander bases” if you choose... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...

...implemented, impacted the entire company. It is important to note that the person who submitted the idea...making something the best it can be that it seems impossible to start at all. This is why perfectionists tend...

...these reagents in both plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free...annealing of the two oligos. Figure 1: Components of TnpB, schematic of pKb-TnpB1 and pKb-TnpB2...

...depositor-provided sequences to confirm the correct plasmid components. For more information on this process, see our...After a run on our Illumina MiSeq, the data is imported into our assembly pipeline that runs with Seqera...

... perfect Our first tip, and arguably the most important, is to take some time to sit down, plan your cloning... plates of frozen glycerol stocks. It is very important that these stay frozen at -80 °C, since multiple...

...select for your vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes...Finally, in some cases a researcher may choose not impose selection on cells and instead to use a vector ...

...timetable for the Singletons in their bins.  The next component of Binning Singletons is when we bring all the...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through meetings...

...toxin-antitoxin roles. Restriction modification systems are composed of restriction endonucleases and methyltransferases...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...

... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...fluorescent proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags. Indeed,...

...Collection aka ATCC where I work. It is virtually impossible to work in cell culture, microbiology, or virology...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

...fusion based on your experimental needs. Equally important, before cloning you must consider whether and ...subcellular environments, including the organelles that compose the secretory pathway, endocytic vesicles, mitochondria...

... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...

...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...Ashley Waldron The mammalian nervous system is composed of myriad cell types and subtypes, and a familiar...

...plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling...sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a binding affinity...

...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in...Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat...

...generations of scFvs against two antigens that are impossible to generate scFvs against in vivo: antigens found...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ...

...hands, these combined pitfalls made it virtually impossible to find the 0.2-0.5% correctly edited alleles...to direct the zygosity of the cells. This was important for us because the Alzheimer mutations we study...

...Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation...21408-21413. PUF Assembly Kit PUF proteins are composed of repeat segments that can be engineered to bind...

...which could potentially truncate the template). Importantly, you also need a DNA polymerase that produces...resulting construct. In cases where the functionally important regions span several kb (or are GC-rich and therefore...