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We narrowed to 760 results for: mpo

Showing: 481 - 500 of 760 results

  1. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...fluorescent proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags. Indeed,...

  3. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...depositor-provided sequences to confirm the correct plasmid components. For more information on this process, see our...After a run on our Illumina MiSeq, the data is imported into our assembly pipeline that runs with Seqera...

  5. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...select for your vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes...Finally, in some cases a researcher may choose not impose selection on cells and instead to use a vector ...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... in a particular organism. Why is codon usage important? Translation and protein synthesis relies on transfer...(Mauro and Chappell 2014).  Ultimately, it is important to take a close look at your experiment and gene...

  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... example all C’s will edit to T’s. Thus, it’s important that there are no “bystander bases” if you choose... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...

  8. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... passive mode, the stronger is the depressive component of the animal behavior. With the availability ...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

  10. The Challenges of Cell Culture

    Type
    Blog Post
    ...Collection aka ATCC where I work. It is virtually impossible to work in cell culture, microbiology, or virology...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

  12. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

  13. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...

  14. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...Ashley Waldron The mammalian nervous system is composed of myriad cell types and subtypes, and a familiar...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in...Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat...

  16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...generations of scFvs against two antigens that are impossible to generate scFvs against in vivo: antigens found...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ...

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling...sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a binding affinity...

  19. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation...21408-21413. PUF Assembly Kit PUF proteins are composed of repeat segments that can be engineered to bind...

  20. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...which could potentially truncate the template). Importantly, you also need a DNA polymerase that produces...resulting construct. In cases where the functionally important regions span several kb (or are GC-rich and therefore...
Showing: 481 - 500 of 760 results