...transition out of the academic sphere into a different meaningful career in science. Supervisors in the academic...scientific backgrounds. For a stated purpose of transitioning a scientist from academia to industry, you might...although most scientist coaches can help with transitioning out of academia (because so many scientists ...Website Check out Our Career Page for Recent Job Openings Find Plasmids to Expedite Your Research   ...

...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...alternative solutions to demonstrate thorough thought planning on the matter. The more detail (within the page...Application" by Vid Mohan-Ram in Science Careers "Beginnings - How to write your first grant proposal" by ...

... allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation of...important to test different lengths and sequences, as unintended secondary structures in your pegRNA could inhibit...3′ end to protect them from degradation. When designing epegRNAs with large structured motifs like mpknot...

... calcium binding proteins (e.g., calmodulin or troponin C). Their fluorescent readouts are used to monitor...can be delivered to intact tissues and organs noninvasively and target a defined population of cells (e....to endogenous calmodulin. Multiplexing Are you combining your calcium reading with other measurements, ... is a FRET-based calcium reporter derived from troponin C. It is engineered with a reduced number of calcium...

...Gate Assembly for cloning, you might be familiar with the rules of thumb for designing your overhang sets...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest ...

... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle...known to cause respiratory diseases in humans, earning this virus a BSL-2 classification. Lentivirus (... can be used for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge...trained in each new place (and pay attention to the trainings!), even if you know guidelines for best practices...

...assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded... is sufficient to disrupt dimerization while maintaining the bright and stable fluorescence of the original...also named their variant mStayGold. It is worth mentioning that Kiryl Piatkevich and colleagues generated... Figure 3: Overview of library generation and screening that led to identification and characterization...

...or failure and demands attention, practice, and honing of your skills from the start. Just like with any...are unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor...may make your perfectly reasonable paragraph unintelligible to a lay audience. My lay paragraph typically...a Grant Writing Professional Facilitate Career Planning and Personal Development with an IDP Get Tips ...

...broke the PE efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized...and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...postmortem brain samples. Results show positive staining in tissues from patients who had confirmed neurodegenerative...

.... P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (2015). An updated... scale that other Cas effectors can’t, while maintaining relatively high efficiency. These alterations... boundaries defined by anti-CRISPR proteins or running into other proteins naturally bound to the DNA....

...after initial mouse training. Other techniques require more skill and additional training, going from intermediate...All researchers must go through the proper basic training prior to conducting mouse experiments. Some of...preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge...

...project was spent selecting guides, designing primers, and running analyses. New team members have taken...guiding principles that we've followed since the beginning. It's free and open to all. The project is funded...blog posts Learn about some considerations when designing gRNAs Find other CRISPR software Resources on...

...Cas9s from other species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in ...SpCas9-VPR in activating transcription. Sites containing NG, GAA, or GAT PAMs averaged 56-91% transcriptional...improved, with a smaller increase in NGA editing. Adenine base editor xCas9 3.7-ABE also follows the same... at an NGG PAM (69 vs 48%) compared to SpCas9-containing ABE7.10, as well as improvements at NG and GAT...

... cells, such as those within hair follicles and lining the digestive system, causing discomfort and illness... enabled researchers to begin discovering and designing molecules that are showing some promise in their...collection is a set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that... Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...

...DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But what about...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification... genes and can therefore be cultured in media containing half the normally recommended antibiotic concentration...plasmid/strain combination individually, based on determining the cell density or by harvesting and miniprepping...

...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional... clone your gene of interest into the multiple cloning site up or down stream of the IRES or 2A element...you can start with a plasmid that has multiple cloning sites flanking the multicistronic element(s), or...Addgene's Plasmid Guide for more molecular biology and cloning information. ...

...to COVID-19. In our lab, we were interested in examining the effect of COVID-19 on worldwide scientific...cases being reported (right, red), likely due to quarantining {% video_player "embed_player" overrideable...candidate at MIT. His research uses applied machine learning and statistical methods for fundamental scientific...interested in using selection, evolution, and machine learning to solve difficult problems in chemistry and chemical...

... misconception. I find personal bias often unintentionally plays a big role in what people think of as...as "the best way" to communicate their science, meaning that they will heavily evangelize for the mode ...cost or freeware alternatives and how steep of a learning curve it will be to get to the necessary level...Static. Often requires both artistic and scientific training. Cartoons may be read as “for children” or may...

...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...insertions (usually via seed growth on antibiotic-containing media) A similar process occurs in nature; ...plants should show no signs of stress, such as anthocyanin production, or disease, such as fungal growth... in a transformation in another 24 hours. The remaining starter culture can be used for colony PCR and...

...search results: More than 200 different GCaMP containing plasmids are available for distribution. If ...already created and deposited it with Addgene. Combining search terms with filters lets you drill down ...) Vector Type: What type of experiment are you planning? (e.g. Retroviral) Species: Specify the source...bacterial resistance marker used to grow plasmid containing bacterial cultures Bacterial Resistance: Use ...