We narrowed to 976 results for: nin

...broke the PE efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized...and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...postmortem brain samples. Results show positive staining in tissues from patients who had confirmed neurodegenerative...

.... P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (2015). An updated... scale that other Cas effectors can’t, while maintaining relatively high efficiency. These alterations... boundaries defined by anti-CRISPR proteins or running into other proteins naturally bound to the DNA....

...after initial mouse training. Other techniques require more skill and additional training, going from intermediate...All researchers must go through the proper basic training prior to conducting mouse experiments. Some of...preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge...

...Cas9s from other species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in ...SpCas9-VPR in activating transcription. Sites containing NG, GAA, or GAT PAMs averaged 56-91% transcriptional...improved, with a smaller increase in NGA editing. Adenine base editor xCas9 3.7-ABE also follows the same... at an NGG PAM (69 vs 48%) compared to SpCas9-containing ABE7.10, as well as improvements at NG and GAT...

...project was spent selecting guides, designing primers, and running analyses. New team members have taken...guiding principles that we've followed since the beginning. It's free and open to all. The project is funded...blog posts Learn about some considerations when designing gRNAs Find other CRISPR software Resources on...

... cells, such as those within hair follicles and lining the digestive system, causing discomfort and illness... enabled researchers to begin discovering and designing molecules that are showing some promise in their...collection is a set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that... Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...

...DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But what about...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification... genes and can therefore be cultured in media containing half the normally recommended antibiotic concentration...plasmid/strain combination individually, based on determining the cell density or by harvesting and miniprepping...

...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional... clone your gene of interest into the multiple cloning site up or down stream of the IRES or 2A element...you can start with a plasmid that has multiple cloning sites flanking the multicistronic element(s), or...Addgene's Plasmid Guide for more molecular biology and cloning information. ...

...characteristic absorbance spectra, which you can detect by examining the A260/280 and A260/230 ratios.  In common practice...you are purifying plasmid DNA from bacteria or cleaning up a PCR product, a common material you are trying...complementary assay, such as a fluorescence based assay or running an agarose gel. In the case of plasmids, the use...allow, you might be better off starting from the beginning. Before trying again, revisit your purification...

... misconception. I find personal bias often unintentionally plays a big role in what people think of as...as "the best way" to communicate their science, meaning that they will heavily evangelize for the mode ...cost or freeware alternatives and how steep of a learning curve it will be to get to the necessary level...Static. Often requires both artistic and scientific training. Cartoons may be read as “for children” or may...

...to COVID-19. In our lab, we were interested in examining the effect of COVID-19 on worldwide scientific...cases being reported (right, red), likely due to quarantining {% video_player "embed_player" overrideable...candidate at MIT. His research uses applied machine learning and statistical methods for fundamental scientific...interested in using selection, evolution, and machine learning to solve difficult problems in chemistry and chemical...

...type of interest. Repeat rounds of infection and screening by PCR enrich for capsid variants with increased...single-cell anatomical study and neuronal tracing, aka determining where a neuron’s axons and dendrites extend to...brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S’s great neuronal transduction...Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...insertions (usually via seed growth on antibiotic-containing media) A similar process occurs in nature; ...plants should show no signs of stress, such as anthocyanin production, or disease, such as fungal growth... in a transformation in another 24 hours. The remaining starter culture can be used for colony PCR and...

...search results: More than 200 different GCaMP containing plasmids are available for distribution. If ...already created and deposited it with Addgene. Combining search terms with filters lets you drill down ...) Vector Type: What type of experiment are you planning? (e.g. Retroviral) Species: Specify the source...bacterial resistance marker used to grow plasmid containing bacterial cultures Bacterial Resistance: Use ...

... and Olsson, 1971), plant lectins (wheat germ agglutinin WGA, Schwab et al., 1978) or fluorescent molecules... mapping of the neuronal circuitry involved in learning and memory (Kohara et al., 2014; plasmids at Addgene...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... motor to auditory pathway important for vocal learning. Nat Neurosci 20(7), 978–986. PubMed PMID: 28504672...Javoy-Agid F., and Agid Y. (1978). Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer...

...are typically lysed in a non-denaturing buffer containing non-ionic detergents, such as NP-40 or triton-X...nonspecific proteins from the sample prior to beginning the IP.  During the IP, the target protein in ...Immunoblotting.) For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively, the protein can ...control, confirming that the capture antibody is functioning as expected. In order to identify any non-specific...

...your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic of the evolution...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies... secreted into the supernatant. Four plasmids containing different nanobodies (NB) were generated in pMAK... dual-deaminase base editor enables concurrent adenine and cytosine editing. Find the plasmids at Addgene...

...E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016) C2c2 is a single-component programmable...Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017... L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional Characterization...Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (2015) Cpf1 Is a Single RNA-Guided ...

...truth is, there is a lot of software development happening at Addgene under the hood. A significant portion...moment a deposit kit is sent to a depositor, to streamlining the order fulfillment process to reduce viral...language and it took us a total of two years, from planning to completion, to do this upgrade while continuing...principles for open science. I hope you enjoyed learning about Addgene’s software development team and ...

...neurotropic variant of AAV9, is better than AAV9 at gaining access to the CNS of C57BL/6J mice following IV...6J mouse strains likely contributed to the unintentionally selection of a virus with mouse-strain dependent... to select novel AAV capsid variants could unintentionally limit the utility of candidate capsids.  Are...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...