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We narrowed to 985 results for: nin

Showing: 481 - 500 of 985 results

  1. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...transition out of the academic sphere into a different meaningful career in science. Supervisors in the academic...scientific backgrounds. For a stated purpose of transitioning a scientist from academia to industry, you might...although most scientist coaches can help with transitioning out of academia (because so many scientists ...Website Check out Our Career Page for Recent Job Openings Find Plasmids to Expedite Your Research   ...

  2. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...alternative solutions to demonstrate thorough thought planning on the matter. The more detail (within the page...Application" by Vid Mohan-Ram in Science Careers "Beginnings - How to write your first grant proposal" by ...

  3. Design Tips for Prime Editing

    Type
    Blog Post
    ... allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation of...important to test different lengths and sequences, as unintended secondary structures in your pegRNA could inhibit...3′ end to protect them from degradation. When designing epegRNAs with large structured motifs like mpknot...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    ...assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded... is sufficient to disrupt dimerization while maintaining the bright and stable fluorescence of the original...also named their variant mStayGold. It is worth mentioning that Kiryl Piatkevich and colleagues generated... Figure 3: Overview of library generation and screening that led to identification and characterization...

  5. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... calcium binding proteins (e.g., calmodulin or troponin C). Their fluorescent readouts are used to monitor...can be delivered to intact tissues and organs noninvasively and target a defined population of cells (e....to endogenous calmodulin. Multiplexing Are you combining your calcium reading with other measurements, ... is a FRET-based calcium reporter derived from troponin C. It is engineered with a reduced number of calcium...

  6. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...Gate Assembly for cloning, you might be familiar with the rules of thumb for designing your overhang sets...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest ...

  7. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle...known to cause respiratory diseases in humans, earning this virus a BSL-2 classification. Lentivirus (... can be used for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge...trained in each new place (and pay attention to the trainings!), even if you know guidelines for best practices...

  8. 6 Tips for Grant Writing

    Type
    Blog Post
    ...or failure and demands attention, practice, and honing of your skills from the start. Just like with any...are unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor...may make your perfectly reasonable paragraph unintelligible to a lay audience. My lay paragraph typically...a Grant Writing Professional Facilitate Career Planning and Personal Development with an IDP Get Tips ...

  9. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...Cas9s from other species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in ...SpCas9-VPR in activating transcription. Sites containing NG, GAA, or GAT PAMs averaged 56-91% transcriptional...improved, with a smaller increase in NGA editing. Adenine base editor xCas9 3.7-ABE also follows the same... at an NGG PAM (69 vs 48%) compared to SpCas9-containing ABE7.10, as well as improvements at NG and GAT...

  10. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...over the previous best performer mGold — while maintaining high brightness and fast maturation time (Figure...TnpB has found itself in the spotlight. TnpBs are gaining popularity, especially among plant biologists, ...barcoding libraries By Mike Lacy Whether you’re planning on lounging poolside or swimming laps, Addgene...allows components to be swapped out, potentially broadening its applications. Find ATLAS plasmids here! ...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...broke the PE efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized...and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...postmortem brain samples. Results show positive staining in tissues from patients who had confirmed neurodegenerative...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    .... P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (2015). An updated... scale that other Cas effectors can’t, while maintaining relatively high efficiency. These alterations... boundaries defined by anti-CRISPR proteins or running into other proteins naturally bound to the DNA....

  13. Common Injection Routes in Mice

    Type
    Blog Post
    ...after initial mouse training. Other techniques require more skill and additional training, going from intermediate...All researchers must go through the proper basic training prior to conducting mouse experiments. Some of...preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge...

  14. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...project was spent selecting guides, designing primers, and running analyses. New team members have taken...guiding principles that we've followed since the beginning. It's free and open to all. The project is funded...blog posts Learn about some considerations when designing gRNAs Find other CRISPR software Resources on...

  15. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ... cells, such as those within hair follicles and lining the digestive system, causing discomfort and illness... enabled researchers to begin discovering and designing molecules that are showing some promise in their...collection is a set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that... Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...

  16. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But what about...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification... genes and can therefore be cultured in media containing half the normally recommended antibiotic concentration...plasmid/strain combination individually, based on determining the cell density or by harvesting and miniprepping...

  17. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional... clone your gene of interest into the multiple cloning site up or down stream of the IRES or 2A element...you can start with a plasmid that has multiple cloning sites flanking the multicistronic element(s), or...Addgene's Plasmid Guide for more molecular biology and cloning information. ...

  18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...to COVID-19. In our lab, we were interested in examining the effect of COVID-19 on worldwide scientific...cases being reported (right, red), likely due to quarantining {% video_player "embed_player" overrideable...candidate at MIT. His research uses applied machine learning and statistical methods for fundamental scientific...interested in using selection, evolution, and machine learning to solve difficult problems in chemistry and chemical...

  19. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... misconception. I find personal bias often unintentionally plays a big role in what people think of as...as "the best way" to communicate their science, meaning that they will heavily evangelize for the mode ...cost or freeware alternatives and how steep of a learning curve it will be to get to the necessary level...Static. Often requires both artistic and scientific training. Cartoons may be read as “for children” or may...

  20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...type of interest. Repeat rounds of infection and screening by PCR enrich for capsid variants with increased...single-cell anatomical study and neuronal tracing, aka determining where a neuron’s axons and dendrites extend to...brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S’s great neuronal transduction...Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...
Showing: 481 - 500 of 985 results