We narrowed to 658 results for: otos

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...their own research. Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been recognized ...

2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...University has used Arch (green-light activated proton pump) and ChR2 (blue-light activated cation channel...

3. Summer SciComm: Preprints

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   ... CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license...

4. Antibodies 101: Selecting the Right Antibody

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   ...Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto T (2016) A proposal for validation of antibodies...

5. Addgene: The First Twenty Years

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   ... the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical School....

6. Antibodies 101: Fab Fragments

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   ...https://doi.org/10.1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M,...

7. All in a Twist: dsRNA

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   ... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

8. dTAG - You're it!

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   ...locus-specific knock-in of FKBP12F36V. Detailed protocols describing the use of these plasmids are available...

9. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...which is necessary for effective STED), and be photostable over a broad pH range. The four biometric probes...

10. Plasmids 101: Golden Gate Cloning

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    ...in a single reaction. For detailed Golden Gate protocols, complete with helpful tips and tricks, see The...

11. 5 Steps to Writing Grant Proposals for Grad Students

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    ...explains 1-2 potential pitfalls of the intended protocol, and offers alternative solutions to demonstrate...

12. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...particular tool has gained widespread popularity – and notoriety – as a tool of choice in the field: dCas9 fused...

13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...protein-antigen complexes will be washed away during the protocol. The amount of antibody that binds in the well...

14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...reproducibility. Non-recombinant antibodies are notorious for being the source of irreproducible data (Begley...

15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...

16. Overcoming the Challenges of Lentiviral Production

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    ...and encourage you to check out the lentiviral protocols and blog posts on Addgene’s website for additional...

17. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...sfCherry2 sfCherry2(1-10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1-10) Improved split...

18. A Look at Addgene's QC Process

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    ...Educational Resources Sequence Analysis of a Plasmid Protocol ...

19. Hot Plasmids February 2024

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    ...Ando et al., 2023). This variant has similar photostability and brightness compared to the original, though...

20. Making CRISPR Plasmids Using Fragmid

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    ...overhang specific to the type of module it is (Pol 2 protomer, Cas protein, ect...). Whatever individual components...