We narrowed to 724 results for: ACE

1. Some Like it Hot: Thermostable GeoCas9

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   ... AA PAM Sequence NGG CRAA (R = A or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal...

2. SunTag and Fluorescent Imaging

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   ...thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum et al. observed...

3. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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   ...improved feature detection with an easy to use interface. We regularly use Snapgene for our quality control...

4. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   ...and offers a high degree of control over time and space. Zebrafish have become a popular model organism ...

5. Hot Plasmids - October 2022

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   ...of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse...

6. Antibodies 101: Fc Effector Functions

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   ... structure, determines isotypes, and provides a place for secondary antibodies to bind. We also know that...

7. Plasmids 101: Gene, enhancer, and promoter trapping

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   ...the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554...

8. We're Updating Our Hold for Publication Policy

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   ...soon as the paper is published, while giving you peace of mind that your materials will only be made public...

9. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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   ... you’re interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound interesting? Is ...

10. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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    ...can also find ReFigure on Twitter (@re_figure), Facebook, and LinkedIn. Many thanks to our guest blogger...

11. FastCloning: A Newer, Simpler Cloning Technique

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    ...products with DpnI, and allow the digestion to take place for an hour at 37°C. This should be enough time ...

12. Addgene at the ASM Microbe 2016 Conference

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    ...science and resource sharing and was also a great place to catch up with former colleagues! I ran into past...

13. Management for Scientists: Giving Feedback

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    ...but is necessary to be direct and clear. There is place in human discourse for indirect language, but it...

14. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    ...and on social media next week, so follow us on Facebook, Twitter, or subscribe to this blog. Find more...

15. Plasmids 101: Antibiotic Resistance Genes

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    ... more stable than Ampicillin and can be used in place of Ampicillin in most applications. Antibiotics ...

16. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...to increase efficiency of insertion and gene replacement. Care to share your favorite CRISPR tool or resource...

17. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    ... a material, a deposit agreement must be put in place. And subsequently, every time that material is requested...

18. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    ...their journal. The early peer-review attempts took place at the beginning of the 18th century. Yet the peer-review...

19. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    ...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and...

20. Plasmids 101: Origin of Replication

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    ...and all of its control elements. The ori is the place where DNA replication begins, enabling a plasmid...