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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 838 results for: ANG

Showing: 501 - 520 of 838 results

  1. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...which have recognition sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ...and high GC content, but some companies now offer Sanger sequencing conditions which can read through this...

  4. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently correct...the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular...

  5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from...properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. ...

  6. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... The space was modern with clean bold colors of orange and white. The decor was very contemporary and ...hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields of...

  7. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...

  8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    .../fddsv.2022.952326Joubert, M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, ...https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang, YC., Liu, IJ. et al. Development of therapeutic...

  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic sequences determines... ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to correctly read through...

  10. The MTA at Addgene

    Type
    Blog Post
    ...or agreement that states the terms of use for a tangible research material that is shared between two parties... via an online process, though the process has changed a lot over the last twenty years! This has helped...

  11. Hot Plasmids - January 2023

    Type
    Blog Post
    ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...CAR–T cells was recently devised by the Yingxiao Wang lab.  The crux of the system is a set of heat-inducible...

  12. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...

  14. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... reagents readily available. What if that could change? Everyone in science needs both a job and funding...around 100 professional editors who produce a broad range of primary research and review journals across life...

  16. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and allowing ...mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology doesn’t have to...

  19. How Addgene Creates Content

    Type
    Blog Post
    ...posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our...

  20. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... a function in a given process. The process can range from a defined pathway or reaction to a complex ...go fishing! References and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic...
Showing: 501 - 520 of 838 results