...families have been characterized. To date, 83% of archaeal and 45% of bacterial genomes utilize this system...translative technologies. Addgene has empowered researchers to harness previous experimental successes and...CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or application that involves...

...are fewer outside influences on your topics of research or course of action One can focus on science for...flexibility with the academic calendar Academic researchers must be extreme self-starters and especially ...structures are common Small integrated teams, little hierarchy Real diversity and acceptance can be an issue...

... were software tools available to biomedical researchers manipulating DNA sequences on a daily basis, ...increasing popularity of gene synthesis, many researchers now use codon-optimized versions of common coding... so our detection system was enhanced to allow searches for a perfect protein sequence match even when...

...stories all the time. They provide accounts of their research struggles in papers. They recount accomplishments...expanding the impact and appreciation for their research. Long story short, telling a good story is something.... And you won’t need to dive as deep into your research at an outreach event as you might in a scientific...

...over to work on career skills development. Back in March, we posted these 25 great tips for scientists working... compiled lists of grants: Science: Where to Search for Funding The Ohio State University; Graduate... Database: 100 Places to Find Funding For Your Research University of Florida: Training Grants and Other...

...Hot Spring at YNP, and since then, many researchers have searched for novel organisms and enzymes from these...our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...

...requirements. To solve this problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo...efficiency. Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...

...Although plasmids started as a somewhat niche area of research, they are now seen as an ubiquitous tool that ...little bit easier for scientists to conduct their research! Since their discovery in the 1950s, plasmids ...have greatly impacted many areas of biological research and have been key in advancing our knowledge in...

... red light instead of blue, which will allow researchers to independently control the activity of two .... Understanding the brain through optogenetics research Addgene: What are some of the big problems that...Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab Sign ...

...), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles have some advantages...isolation protocol by Maguire and colleagues, other researchers have tried to modify it to improve yields. For... viral protocols and videos Find AAVs for your research ...

... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

...by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types of biological...enough to engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly...environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department of...

...the University of Alberta. If you’re like many researchers these days, you are ready to take (if you haven...are often impractical for small projects. Most researchers instead turn to so-called “mismatch nuclease”...knockout of HIV co-receptor CCR5. Nucleic Acids Research 43, 5560–5571 (2015). PubMed PMID: 25964300. PubMed...

...γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus... 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or drive high...Cycle Popular Retroviral Vectors and Their Use in Research More resources on addgene.org Addgene Protocols...

...base editors for your research Read our CRISPR guide Find plasmids for your research ...compatible with all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a...

... for your experiment. Research applications Gene expression control Researchers are always wanting to ...fact, a choice delivery vector from basic science research all the way to clinical gene therapy. But how ...

...-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing...https://doi.org/10.1074/jbc.m116.736942  National Research Council (US) Committee on Methods of Producing...resource on the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies...

...GPCRs. This collection, called TRUPATH, allows researchers to identify specific G proteins activated by ...s still a lot to learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic...chemogenetics guide Find chemogenetics plasmids for your research ...

...expression of recombinant proteins for industrial or research purposes requires stable host cells (the simplest...commercial operators, government agencies, active researchers, as well as fellow iGEM teams. We were the proud...more productive and collaborative environment for research.  We’ve also involved ourselves in extensive outreach...

...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular biology and...inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM can help...Perfect Plasmid Backbone Find Plasmids for Your Research ...