We narrowed to 712 results for: BASE
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TypeBlog Post... a single allele in a human line, using methods based on homologous recombination (Rago et al., 2007)....opportunities, it’s an exciting time to be in the lab. Based on the power of available techniques and how quick...nuclease cleaves double stranded DNA (dsDNA) at basepair mismatches. To use this tool to detect a BRAF ...strand from the control cells, which should contain basepair mismatches at the site of the edit. Using Surveyor...
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With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...against unnecessary clinic regulations, evidence-based research is vital in combating misinformation and...83.7430°W – Alexandra Greenbaum Science and evidence-based policy and legislation hit home hard in Michigan... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...the authors of these papers did. Since the SunTag-based system tethers mRNAs to the plasma membrane, it’... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...Include follow-up questions or requests for advice based on your discussion. Perhaps you have a paper, protocol... -
Plasmids 101: Origin of Replication
TypeBlog Post...generally high in As and Ts. Why, you ask? Well, A-T base pairs are held together with two hydrogen bonds ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are not suited ... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...and how components of the plasmid should change based on bacteria type. The gram positive and negative... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...Fink, Y., & Anikeeva, P. (2019). Flexible fiber-based optoelectronics for neural interfaces. Chemical ... -
An Addgene Summer Internship
TypeBlog Post...expected something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to... -
The MTA at Addgene
TypeBlog Post...be distributed for research use only to industry-based scientists. Some organizations choose to share their... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post... antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post.... I am the cofounder and CEO of miLab, a Boston-based startup focused on empowering early-stage scientists... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...types and subtypes using polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas... -
Genetic Code Expansion
TypeCollection...Modified Constructs The genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids...acid would be incorporated. Other options, such a 4-base pair codons, have also been utilized. A tRNA that... -
General Transfection
TypeProtocol...will drift pretty rapidly upon addition of acid or base. Add only a few drops at a time, allow them to mix... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...3 nonprofit and our plasmid service was started based on donations. That seed money was used to build ... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...application for OptoNBs takes us into cells: light-based control of signaling pathways. This is particularly... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...0460.1000439 6. Wu et al. 2020. . “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...Found in surgical suites, air fields, and military bases, checklists are the ultimate tool for doing things... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...quickly learned that my approach needed to adapt based on individual needs. For instance, I had one colleague...