We narrowed to 710 results for: BASE;

1. Plasmids 101: Origin of Replication

   Type

   Blog Post

   ...generally high in As and Ts. Why, you ask? Well, A-T base pairs are held together with two hydrogen bonds ...

2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   ...calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are not suited ...

3. Plasmids 101: Gram Negative and Positive Bacteria

   Type

   Blog Post

   ...and how components of the plasmid should change based on bacteria type. The gram positive and negative...

4. Visualizing Translation at the Single Molecule Level

   Type

   Blog Post

   ...the authors of these papers did. Since the SunTag-based system tethers mRNAs to the plasma membrane, it’...

5. Multifunctional Neural Fibers: Modulation from Gut to Brain

   Type

   Blog Post

   ...Fink, Y., & Anikeeva, P. (2019). Flexible fiber-based optoelectronics for neural interfaces. Chemical ...

6. Antibodies 101: Chimeric Antibodies

   Type

   Blog Post

   ... antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma...

7. An Addgene Summer Internship

   Type

   Blog Post

   ...expected something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to...

8. The MTA at Addgene

   Type

   Blog Post

   ...be distributed for research use only to industry-based scientists. Some organizations choose to share their...

9. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   ...Include follow-up questions or requests for advice based on your discussion. Perhaps you have a paper, protocol...

10. Don’t Wait To Launch Your Health Science Startup

    Type

    Blog Post

    .... I am the cofounder and CEO of miLab, a Boston-based startup focused on empowering early-stage scientists...

11. History of CRISPR Cas - A tale of survival and evolution

    Type

    Blog Post

    ...types and subtypes using polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas...

12. Genetic Code Expansion

    Type

    Collection

    ...Modified Constructs The genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids...acid would be incorporated. Other options, such a 4-base pair codons, have also been utilized. A tRNA that...

13. General Transfection

    Type

    Protocol

    ...will drift pretty rapidly upon addition of acid or base. Add only a few drops at a time, allow them to mix...

14. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type

    Blog Post

    ...activation of transcription in bacteria by co-opting σ54-based transcription. Transcription from σ54 promoters ...

15. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type

    Blog Post

    ...0460.1000439 6. Wu et al. 2020. . “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics...

16. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    ...Found in surgical suites, air fields, and military bases, checklists are the ultimate tool for doing things...

17. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post

    ...3 nonprofit and our plasmid service was started based on donations. That seed money was used to build ...

18. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    ...M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated to capsid protein ...

19. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    ...expression.     AAV retrograde Unlike traditional dye-based neuronal tracing reagents, AAV particles require...

20. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type

    Blog Post

    ...consistent with the cell type that was predicted based on the Atlas. They also looked at 17 of these TFs...