We narrowed to 756 results for: ELP

1. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   ...plasmids recently deposited by Chris Voigt’s lab help keep gene expression stable regardless of copy number...

2. dTAG - You're it!

   Type

   Blog Post

   ...disrupting protein function. A literature search can help determine which terminus is favorable for tagging...

3. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   ... TRIM-Away podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression...

4. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   ...ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy technique...

5. Addgene: The First Twenty Years

   Type

   Blog Post

   ...was established as an e-commerce company, which helped it stay ahead of the curve as access to the Internet...

6. Antibodies 101: Fab Fragments

   Type

   Blog Post

   ...components of your experiment. These considerations will help you narrow down which antibody format to use for...

7. Human Germline Editing Using CRISPR

   Type

   Blog Post

   ...gene. Ma et al. claim that CRISPR would still be helpful in increasing viable embryos, especially in the...

8. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...

9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   ...feedback from all members of the research community to help us develop these resources further. Feel free to...

10. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    ...used to ensure specificity; this is particularly helpful when wanting to target specific neuron subtypes...

11. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ...would like. Check out our promoter blog if you need help choosing. Viral expression is another mechanism ...

12. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    ...Gradinaru Labs developed a machine-learning model to help them construct “designer” channelrhodopsins with...

13. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    ...Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as the OG (as ...

14. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    ...Western blot, a secondary antibody is ideal as it will help to amplify the signal. Some applications use either...

15. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    ...other short epitope tags (Kimple et al., 2013) could help improve the transport of protein of interest the...

16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    ...kilobases, but only in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective ...

17. Antibodies 101: Conjugation

    Type

    Blog Post

    ...antibodies in your refrigerator often need a little help to be useful. Mainly because, well, antibodies are...

18. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    ... detailed Golden Gate protocols, complete with helpful tips and tricks, see The Sainsbury Lab website ...

19. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    ...tons of parts and characterizing the software to help you put it together. The software will be available...

20. Engaging with science and society at pgEd

    Type

    Blog Post

    ...preparing you for future work in these areas, and in helping you decide whether you enjoy this kind of work....