We narrowed to 660 results for: EPO

1. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   ...12 years, I’m very familiar with the role our repository has played in these fields, and seeing names ...

2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   ...dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining {%...

3. DNA Purification Without a Kit

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   Blog Post

   ...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...

4. A Guide to Starting Your Own Journal Club

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   Blog Post

   ...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...

5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   ...PFA is dissolved in solution and heated, it depolymerizes, producing formaldehyde. Often people will call...

6. Summer SciComm Series: Modes of Communication

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   Blog Post

   ... videos, and Twitter, for sharing updates, new deposits, and new blog posts!    How to choose  Where...

7. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   ...contamination. However, this ratio can be misleading. Some reports suggest that the A260/280 ratio does not drop significantly...

8. Plasmids 101: Colony PCR

   Type

   Blog Post

   ...Technique Learn to Perform a Bacterial Transformation Deposit Your Positive Clones with Us ...

9. CRISPR Antimicrobials

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   Blog Post

   ...small pools of antibiotic resistant cells could repopulate the environment after treatment. The phagemid...

10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ...get Addgene from a concept to an up and running repository. As they built the foundation for what Addgene...

11. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    ...opportunity deadlines, and we ensure that grant reports are submitted and project evaluations are completed...

12. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

    ...questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage...

13. CRISPR Guide

    Type

    Collection

    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols Video protocol for genomic deletions...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...

14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    ...concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective pressure and...

15. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...

16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    ...correlation coefficient (r) is also computed and reported.  The higher the R2 value, the more confident ...

17. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...astrocytes, and oligodendrocytes, provided that reporter genes specific to these cell types are used. The...

18. Career Insights: Technical Support Specialist

    Type

    Blog Post

    ...certainly there won’t be a shortage of amusing reports where someone tries to “optimize” a particular ...

19. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...highly customizable in terms of how they actually “report” as biosensors. Making aptamers glow The flexibility...

20. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ...scientists around the world have generated and deposited their own Gateway-compatible plasmids with Addgene...