We narrowed to 625 results for: Emb

1. Hot Plasmids - February 2022

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   Blog Post

   ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

2. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

3. Antibodies 101: Isotypes

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   Blog Post

   ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

4. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

5. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

6. Multiplex Genome Editing with CRISPR-Cpf1

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   ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

7. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

8. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

9. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

10. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post

    ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

11. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

12. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

13. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

15. Addgene: The First Twenty Years

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    Blog Post

    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

16. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

17. The time and cost required to make a plasmid

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    Blog Post

    ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

18. Summer SciComm: Preprints

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    Blog Post

    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

19. Antibodies 101: Affinity Reagents

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    Blog Post

    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

20. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...