We narrowed to 621 results for: Emb

1. Hot Plasmids - February 2022

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   Blog Post

   ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

2. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

3. Antibodies 101: Isotypes

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   Blog Post

   ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

4. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

5. Viral Vectors 101: Viral Applications

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   Blog Post

   ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

6. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

7. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

8. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

9. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

10. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

11. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

12. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

13. Summer SciComm: Preprints

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    Blog Post

    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

14. Antibodies 101: Affinity Reagents

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    Blog Post

    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

15. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

16. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

    ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

17. Addgene: The First Twenty Years

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    Blog Post

    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

18. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

19. The time and cost required to make a plasmid

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    Blog Post

    ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

20. Choosing the B(right)est Fluorescent Protein: Photostability

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    Blog Post

    ...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...