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We narrowed to 802 results for: Eng

Showing: 501 - 520 of 802 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty of sharing...into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques are neither...

  2. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do a lot of genetic construction...experiments. Not all of our lab enzymes are made by New England BioLabs, but the NEB Tools app comes in handy when...

  3. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... Complementary to the released strand, 127bp in length, With 36bp on the PAM-distal side of the break ...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  4. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...comments section below! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources...

  5. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...scientists and technical people) is linked to engagement and access to growth opportunities, not salary...zone when you lose track of time because you are engrossed in a task. Things seem to fall into place frequently...

  7. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...Even if you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply...Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources at Addgene.org...

  10. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small molecules to...Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of the human muscarinic receptor...

  11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses? There...There are a few important differences between engineered γ-retroviruses and lentiviruses. First, these viruses...

  12. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex...spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA?...

  14. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Campus and the University of California, Berkeley engineered an AAV variant that efficiently infects neurons...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...

  15. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...typically their own genome, but this step can be engineered to be anything you want delivered to your target...and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift mutations in genes of...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...routine, open-source software projects that actively engage with the community are particularly successful....hardware, this process is often much more involved. Challenges such as the provision of an accessible supply...

  17. Clear and Accessible Writing

    Type
    Blog Post
    ...scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...dive into applying these four steps elsewhere, engaging in this practice, and perhaps modifying it as ...

  19. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide, resulting in...Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed...

  20. To Each HIS Own

    Type
    Blog Post
    ...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...
Showing: 501 - 520 of 802 results