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We narrowed to 1,145 results for: Gene

Showing: 501 - 520 of 1145 results

  1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post
    ...which NANOG-related genes are critical to stem cell survival. Knowing which genes influence the survival...compaction, an enhancer can increase the expression of a gene that is millions of base pairs away. (For details...GPS for the genome” to compute coordinates for genetic elements like protein, RNA, and DNA (Table 1). ...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...differentiate over time, we periodically use next-generation sequencing to measure the relative proportion...pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing your own CRISPR...Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS...

  2. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post
    ...versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed in the...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited attitude...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...That so many scientists are tackling this type of genetic engineering is a testament to how amazing the downstream...from the custom software used to design complex genetic circuitry to the downstream output. Tuesday morning's...

  3. London Calling - Addgene’s New Europe Office in London

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    Blog Post
    ...contacting Addgene can be found on our Contact page. You’ve called. You’ve emailed. And Addgene has been...Plasmid sharing in Europe: Addgene’s request statistics Since 2004, Addgene has shipped more than 75,000...into the Addgene repository. More than 4,500 plasmids have already been deposited at Addgene by over 250... your service: Addgene’s London office Just a few weeks ago I became the newest Addgene Outreach Scientist...UPDATE - As of January 2020, Addgene's Europe office no longer handles customer support inquiries. The...Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office located in London...poised and ready to answer your questions about Addgene, plasmids, and depositing (all while our US counterparts...

  4. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    Blog Post
    ...frequently requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was...expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering methods improved...Addgene was founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids...biosensors, optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium...Top 10 plasmid technologies distributed through Addgene over our first 10 years. 10 most distributed plasmid...it's not actually a technology, but pSV-PGC1 was Addgene's first distributed plasmid and was created in the...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid...

  5. Addgene’s New Way to Help You Share Science

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    Blog Post
    ...When I got the job of Outreach Scientist at Addgene, my first thought was “I get to talk to scientists... spend a lot of time telling scientists about Addgene’s repository, and explaining how to deposit and ... 1,600+ labs around the world!) that have kept Addgene at the forefront of life sciences research for ...from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and get practical...scientific sharing and the open-access movement. At Addgene, we believe that science gets better when everyone... to tell? Let us know by emailing us at blog@addgene.org or tell us on Twitter and Facebook. We’ll track...

  6. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

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    Blog Post
    ...Projects in Wei Leong Chew's lab. Genome engineering, gene delivery methods, AAVs, and more. Chapter 7/Outro... in the areas of genome editing, immunology, and gene therapy.   Special thanks to our sound engineer/...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids...synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology ... addgene podcast...

  7. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

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    Blog Post
    ...international researcher who uses plasmids containing genes from rabies and vesicular stomatitis virus, you ... export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster...ordering plasmids, please feel free to email help@addgene.org. We’ll be happy to help! A quick note: While ...complex and may change suddenly. Please email help@addgene.org for the most-up-to-date information on shipping...

  8. Using AAV for Neuronal Tracing

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    Blog Post
    ...Hybrid Serotypes: Improved Vectors for Gene Delivery. Curr Gene Ther 5(3), 299–310. PubMed PMID: 15975007...vectors could also be powerful tools for the field of gene therapy if they can reach the affected part of the...anterograde and retrograde axonal transport. Hum Gene Ther 25, 705–720. PubMed PMID: 24694006. PubMed ...2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems...., and Nakahara, K. (2011). Local and retrograde gene transfer into primate neuronal pathways via adeno-associated...-associated viral vectors is serotype-dependent. Gene Ther 20(3), 348–352. PubMed PMID: 22418061. PubMed...vector serotypes 1, 2, 5, 7 and 8 in mouse brain. Hum Gene Ther 18, 195–206. PubMed PMID: 17343566. Tervo, ...

  9. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post
    ...sequence to confirm backbone elements.   Confirming the gene/insert by aligning to NCBI entry or using BLAST....available reference sequence or plasmids containing genes with many modifications. We look for point mutations...amino acid. We also confirm that the species of the gene matches the data associated with the plasmid.   ...Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum...for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control...control for over ten years. Now, thanks to a generous donation by the Hirsh family, we are able to take...provide requesting scientists high quality data! Addgene's new in-house NGS process To start, the process...

  10. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...control expression of their genes of interest. While recombinases are powerful gene expression tools in and...CRISPR knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the ...blue-light inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases are enzymes...precisely controlled through the use of optogenetics. Optogenetic tools use light to induce conformational...Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting...from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna...unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion...

  11. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

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    Blog Post
    ...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...we know biological resource centers, including Addgene, positively affect scientific research. As Maria...Maria Castro of University of Michigan says, “Addgene has transformed the way in which we share plasmids...tool is so influential that it is requested from Addgene many times as the technology disseminates throughout...researchers who have at least one plasmid deposited at Addgene that has been distributed more than 100 times. ... honor for our powerhouse depositors who have generously shared their reagents freely with the research... on sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators...

  12. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post
    ...data Do you use Addgene AAV tools and want to join the ranks of our growing group of Addgene AAV data sharing...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re... way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot in 2019 as... an open science platform for scientists using Addgene AAV viral preps to easily find and share useful...animal strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over...sharing champions? If you’ve recently used Addgene AAV tools, we invite you to contribute your data to ... for sharing on the site. The entry form has 5 general question types and it takes approximately 15 minutes...

  13. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

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    Blog Post
    ...that, when you cite a plasmid from Addgene using its unique Addgene ID number, your publication will be...Feature Browse More Inside Addgene Posts Additional Resources on Addgene.org Browse Our Special Collections...plasmid technologies contributed by our depositors, Addgene strives to make it easier for you to find the plasmids...sophisticated search, and curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you...repository, we request that you cite it using both its Addgene ID number as well as the reference for its original... process, we explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation...Why do this? Not only does citing plasmids from Addgene help others find them later if they need to use...

  14. More Data for You: Find Articles Citing Addgene Plasmids

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    Blog Post
    ...with this "Citing Article" - such as cloning their gene of interest into a previously deposited vector -...Plasmid" list. Email us at help@addgene.org with the Plasmid Name, Addgene Plasmid ID #, and the PubMed ...ICYMI: Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...systems it has been validated in.  If a plasmid's Addgene ID # has been referenced in other publications,... see where they've been cited. Benefits to the Addgene community of scientists Benefits to depositing ...Properly citing plasmids you've requested from Addgene may get your paper listed in the "Articles Citing...

  15. From our Table to Yours: An Inside Look at Lunch at Addgene

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    Blog Post
    ...sharing Addgene is all about helping scientists share between labs, and people work hard at Addgene to make...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can only provide... started Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams...a year, she’ll mix up the names of everyone at Addgene, and send random sets of 4 people out to lunch.... to gather around a large table to eat with my Addgene family every day. Do you have any traditions that...

  16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    Blog Post
    ...and topology, and cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook... as everything from parasites, to symbionts, to genes and the labels applied to them were vague or contradictory...biology was never the same. Over the next few years genes from different bacterial (and eventually mammalian...Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic...of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant...separate term, “episome”, defined as “a non-essential genetic element which could exist either autonomously or...molecular biologists wanting to study bacterial genetics. This all changed thanks, in part, to a collaboration...

  17. Top Requested Plasmid of 2017 - pMD2.G

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    ...many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested...means researchers who use VSV-G can deliver their genes of interest to a variety of cells and study their...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours ... articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...Lentiviral Transduction Additional Resources on Addgene.org Check out our Viral Service Learn more about...

  18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post
    ...on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find ...examine a dataset of Liu lab plasmid requests from Addgene, as a proxy for global activity in our particular...over 6 years for 35 papers (data was provided by Addgene on 5/26/20 and contains requests from 5/22/14 to...The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect, though...not a causal interpretation -- the effect would generally not be the same if weekends were somehow imposed...evolution to illuminate biology and enable next-generation therapeutics. Prime editing, base editing, PACE...

  19. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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    Blog Post
    ...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists... this alone. If you have deposited plasmids at Addgene and would like to make them available to industry...industry, please reach out at techtransfer@addgene.org and we will initiate a conversation with you and your...

  20. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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    Blog Post
    ...features of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...Did you see Blugene’s make-over and our new homepage? If you've visited Addgene’s homepage recently, ...been working on behind the scenes here at Addgene.  Addgene's new homepage While users are increasingly...emphasize the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on....  Blugene gets a make-over You may also have noticed another big update on the homepage. Blugene, our... ways, we realized that Blugene needed the same thoughtful treatment. Blugene has been with us since 2011...might notice Blugene looking sad, especially if an experiment doesn’t go as planned. Blugene also has full...
Showing: 501 - 520 of 1145 results