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We narrowed to 911 results for: IRE

Showing: 501 - 520 of 911 results

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...biological phenomena by generating an output which is directly proportionate to the amount of a given factor ...either factors as proxies for a cellular process or directly measure a cellular process. Cellular phenomena...type of cells the viral particles infect. This can direct your biosensor to specific tissues or cell (sub...

  3. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...transfect’, such as primary cells. They can also be directly injected into hard-to-reach organs, such as the...like replication incompetent viral particles, are required. We will be covering these safety measures in ...dividing cells, as their access to the host genome requires breakdown of the nuclear envelope, which occurs...

  4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...tropism. Briefly, different envelope plasmids can direct the production of virus with various tropisms.  ...therefore, reduces the number of plasmids that are required in the transfection step of the viral packaging...incompetent? Yes, as with lentiviruses, the genes required for γ-retroviral packaging and transduction are...

  5. How Addgene Creates Content

    Type
    Blog Post
    ...truly believes that sharing speeds science, and our entire mission is to enable that process. Our repository... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, for example,...

  6. RNA Extraction Without A Kit

    Type
    Blog Post
    ...RNA, a fast, strong protein denaturant would be required- something that broke down RNases before RNases... effective at isolating total RNA, this method requires a lot of time and depending on how many samples...without a kit!) As mentioned above, working with RNA requires keeping your samples cold until homogenization...

  7. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...to read a formulaic fiction story that doesn’t require any detailed retention of information or formulation...something verbally. Sometimes a group was just really tired and needed a slower, simpler presentation than I... I had planned; other times students were fired up and enthusiastic and needed a faster presentation with...

  9. To Each HIS Own

    Type
    Blog Post
    ...Much of today's biological research requires a close examination of specific proteins within a system...tags, however, tended to be very large and often required the incorporation of a cleavage site and a post-purification...no cut or dried answer. Successful purification requires the tag to be accessible to the metal ions once...

  10. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...sufficient for certain patterning activities of entire tissue types or even organs. In frogs, ectopic ...electrical communication is briefly inhibited in the entire worm during regeneration, the worm creates two ... artificial intelligence," says Michael Levin, Director of the Allen Discovery Center. The future of this...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...sex, the National Institutes of Health (NIH) now requires that sex be included as a biological variable ...variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on Women’s Health, emphasizes...gender in science and technology and currently directs the EU/US Gendered Innovations in Science, Health...

  12. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...with multiple inserts or internal mutations that require more than two sequencing reactions. In the more...to ensure uniformity of the culture. Sequencing directly from the glycerol stock saves us the step of purifying... Each sequencing service will have different requirements for volume and concentration, and not all providers...

  13. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...new job skill? Finish your thesis? Knowing your desired outcomes will help you assess if the coach you ...coach usually will be a clear communicator and make direct suggestions for improvement and change. Work ...relationship has been successful – what are the desired outcomes? Ask openly for clear information on...

  14. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...asymmetry in the core sequence gives the loxP site directionality, and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT...reversed.   Deletion: If the sites face in the same direction, the sequence between the loxP sites is excised...cerevisiae FLP-FRT recombination system is another site-directed recombination technology very conceptually similar...

  15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ..., comprised of PAS and GAF domains of iRFP713. Requires presence of heme oxygenase for chromophore formation...miRFP709 and miRFP670, pair with common PAS domain. Requires presence of heme oxygenase for chromophore formation...BiFC interaction is typically irreversible and requires some time for chromophore maturation, splitFAST...

  16. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is... is expected to contribute to experiments that require high photostability, such as time-lapse imaging...Leopold Parts. This library delivers 2 randomly paired guides per construct and allows a reduced scale...

  18. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative data on all the cell divisions of ...Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen lab. SapTrap...Project: The Complete Gene Expression Map of an Entire Nervous System." Neuron 99.3 (2018): 430-433. PubMed...

  19. CRISPR in the Clinic

    Type
    Blog Post
    ...Systemic therapies involve CRISPR-Cas delivery directly to the patient. Patient-derived cells can alternatively...the goal of ‘fixing’ mutations or introducing a desired edit, but this particular class of treatments has...inflammatory diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR...

  20. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...longer incubation times and didn’t necessarily require cycling. The Pros and Cons Researchers used the...select for correctly assembled phage genomes, it required a very stringent selection process and benefited...selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While the 52-piece...
Showing: 501 - 520 of 911 results