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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 808 results for: MAK

Showing: 501 - 520 of 808 results

  1. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...     The Trimmer Lab has continued to work to make the sequences of monoclonal antibodies publicly ...sequences from the Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded...

  4. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...testing and a lack of well-characterized genetic tools make it challenging to engineer a specific function in...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...

  5. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...systemically administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory...delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used in research...

  6. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express...

  7. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...exposure to autophagy-inducing conditions. Rosella makes it easier to study the mechanisms behind autophagy... shown in Sargsyan et al. It is also possible to make Rosella fusions and thereby monitor autophagy of...

  8. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...However, they note that certain improvements could make CRISPRainbow even better. Adding an additional hairpin...activation/repression and genome editing. Ready to make your cells a little more colorful? CRISPRainbow ...

  11. Summer SciComm: Preprints

    Type
    Blog Post
    ...comment online! Want the general public (say, policymakers or community organizations) to use your work...those plasmids alongside a journal article, but it makes the research materials available to the community...

  12. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...time to read the book.  Intuitively, this probably makes sense. It’s a lot easier to read a formulaic fiction...happens to us all!) and offer a variety of options to make your content more accessible to your audience.  ...

  13. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...short linker peptide. The lack of constant region makes them less immunogenic, but they have lower affinity...produced in bacteria and are generally cheaper to make than antibodies. Minibodies are created by binding...

  14. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

  17. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — ...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...

  20. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...to CRISPR experiments, therefore, you often must make a choice about what is your top priority: location...trying to knock-in a patient mutation or need to make a precise amino acid change, there is very little...
Showing: 501 - 520 of 808 results