We narrowed to 775 results for: Mos

... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...

...several sgRNA’s at the same time, and choose the most effective ones to increase your chances of gene ...the pronuclei were fusing, you may have several mosaic mice. To figure out what alleles are present in...

...included two reference tables below listing some of the most common bacterial and mammalian promoters. These ...mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less active...

...neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors...pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1...

...RNA genomes into DNA to be integrated into the chromosome of the cell. This integrated viral genome is ...than regular lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from...

...labeling cells for flow cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated...antibodies but on small molecule dyes, are useful, most notably when it comes to viability and proliferation...

... will be represented through FSC-A vs. SSC-A, so most people use that setting. Figure 2: As the...the two cell types (likely B cells and others). Almost no cells stain positively for both markers. When...

...premature aging, and other harmful processes. The most dangerous type of DNA lesion results from breakage...double-strand break repair and their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (...

... Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting as a full-fledged...Containing the spread of this virus has become an utmost priority for countries around the world and to ...

...scientists using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable...2014). While 10 derivatives of TO exist, Mango is most frequently used with TO1-biotin or TO3-biotin (the...

...are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength to use but can...publication, including the supplemental data. You’re most likely to find thorough characterization data, like...

...several ways to achieve multiplex IF. One of the most common approaches is to use sequential cycles of...are a few specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies...

...research is needed to determine which recA- strains are most effective at reducing multimerization and define...of Multimeric Forms of Circular Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128...

...like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas of the membrane... x-ray film or with specific imaging devices. In most cases, the primary antibody, which binds the protein...

...works best with insertions of 2 kb or less. Luckily, most of the commonly used cassettes, such as fluorescent...independent core facilities for 13 projects, with most projects requiring only 40-60 zygotes to generate...

...repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing system derived... conventional methods. First, one can work with almost any strains of mice, as compared with conventional...

... very useful for genetic engineering approaches. Most importantly for this technology, however, is that...over transfecting with plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike...

...Using a DNA template for the RNAi trigger is the most prevalent method due to ease of use and capacity...actively silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly ...

...expression in the retina and in parts of the brain, but almost none in the liver (A). In general, RNA levels tend...analyze the resulting data, so it may not be the most accessible option for many laboratories. Additionally...

... is a powerful experimental strategy, one of the most common concerns we get here at Addgene about this...-homologous pairs) (Figure 2.). They found that almost all off-target expression can be attributed to ...