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We narrowed to 765 results for: Mos

Showing: 501 - 520 of 765 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...several sgRNA’s at the same time, and choose the most effective ones to increase your chances of gene ...the pronuclei were fusing, you may have several mosaic mice. To figure out what alleles are present in...

  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...included two reference tables below listing some of the most common bacterial and mammalian promoters. These ...mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less active...

  4. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...RNA genomes into DNA to be integrated into the chromosome of the cell. This integrated viral genome is ...than regular lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from...

  5. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...labeling cells for flow cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated...antibodies but on small molecule dyes, are useful, most notably when it comes to viability and proliferation...

  6. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... will be represented through FSC-A vs. SSC-A, so most people use that setting. Figure 2: As the...the two cell types (likely B cells and others). Almost no cells stain positively for both markers. When...

  7. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...premature aging, and other harmful processes. The most dangerous type of DNA lesion results from breakage...double-strand break repair and their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors...pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1...

  9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting as a full-fledged...Containing the spread of this virus has become an utmost priority for countries around the world and to ...

  10. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...scientists using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable...2014). While 10 derivatives of TO exist, Mango is most frequently used with TO1-biotin or TO3-biotin (the...

  11. Adenovirus Guide

    Type
    Guide
    ...is the most popular method for creating first-generation rAdV vectors, which is still the most commonly...range of mild diseases in humans and other animals, most commonly respiratory infections, such as the common...applications. However, this has also made them the most widely used viral vector platform for vaccine design...vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus type 5 (Ad5...autonomously. First-generation rAdV vectors remain the most popular choice due to their well-established methods...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...plasmid pAdEasy-1 (Addgene #16400) is suitable for most purposes. For especially long transgenes, the use...

  12. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength to use but can...publication, including the supplemental data. You’re most likely to find thorough characterization data, like...

  13. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...several ways to achieve multiplex IF. One of the most common approaches is to use sequential cycles of...are a few specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies...

  14. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...research is needed to determine which recA- strains are most effective at reducing multimerization and define...of Multimeric Forms of Circular Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128...

  16. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas of the membrane... x-ray film or with specific imaging devices. In most cases, the primary antibody, which binds the protein...

  17. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing system derived... conventional methods. First, one can work with almost any strains of mice, as compared with conventional...

  18. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... very useful for genetic engineering approaches. Most importantly for this technology, however, is that...over transfecting with plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...Human embryonic kidney 293T (HEK293T) cells are the most common platform to produce AAV vectors. Additional...genome at a specific site, the AAVS1 site on human chromosome 19. This integration is mediated by proteins ...molecular weight structures that are maintained extrachromosomally. Random integration of rAAV genomes in non-homologous...for the production of capsid proteins. AAV2 is the most commonly used serotype for rAAV vector construction...delivery of CRISPR/Cas9 elements, making it one of the most common methods for in vivo CRISPR-based genome editing...incompetent and not known to cause disease in humans. Most, if not all of Addgene’s transfer plasmids are replication...
Showing: 501 - 520 of 765 results