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We narrowed to 772 results for: Mos

Showing: 501 - 520 of 772 results

  1. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

  2. Plasmids 101: Codon usage bias

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    Blog Post
    ...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...Nonsense! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism...

  3. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...several sgRNA’s at the same time, and choose the most effective ones to increase your chances of gene ...the pronuclei were fusing, you may have several mosaic mice. To figure out what alleles are present in...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...included two reference tables below listing some of the most common bacterial and mammalian promoters. These ...mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less active...

  5. CRISPR 101: Homology Directed Repair

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    Blog Post
    ...premature aging, and other harmful processes. The most dangerous type of DNA lesion results from breakage...double-strand break repair and their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (...

  6. Hot Biosensors 2022: Year-End Roundup

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    Blog Post
    ...neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors...pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1...

  7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...RNA genomes into DNA to be integrated into the chromosome of the cell. This integrated viral genome is ...than regular lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from...

  8. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...labeling cells for flow cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated...antibodies but on small molecule dyes, are useful, most notably when it comes to viability and proliferation...

  9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ... Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting as a full-fledged...Containing the spread of this virus has become an utmost priority for countries around the world and to ...

  10. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ...scientists using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable...2014). While 10 derivatives of TO exist, Mango is most frequently used with TO1-biotin or TO3-biotin (the...

  11. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ... will be represented through FSC-A vs. SSC-A, so most people use that setting. Figure 2: As the...the two cell types (likely B cells and others). Almost no cells stain positively for both markers. When...

  12. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength to use but can...publication, including the supplemental data. You’re most likely to find thorough characterization data, like...

  13. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...several ways to achieve multiplex IF. One of the most common approaches is to use sequential cycles of...are a few specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies...

  14. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...research is needed to determine which recA- strains are most effective at reducing multimerization and define...of Multimeric Forms of Circular Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128...

  16. Antibodies 101: The Basics of Western Blotting

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    Blog Post
    ...like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas of the membrane... x-ray film or with specific imaging devices. In most cases, the primary antibody, which binds the protein...

  17. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post
    ...repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing system derived... conventional methods. First, one can work with almost any strains of mice, as compared with conventional...

  18. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ... very useful for genetic engineering approaches. Most importantly for this technology, however, is that...over transfecting with plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike...

  19. Antibodies 101: Validation

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    Blog Post
    ...expression in the retina and in parts of the brain, but almost none in the liver (A). In general, RNA levels tend...analyze the resulting data, so it may not be the most accessible option for many laboratories. Additionally...
Showing: 501 - 520 of 772 results