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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 876 results for: NSI;

Showing: 501 - 520 of 876 results

  1. Fluorescence Titering Assay

    Type
    Protocol
    ...applications. Safety Warnings Lentivirus is generally considered biosafety level 2+. Please ensure that you are... in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health of the target cell... pipetting or inverting. Aliquot 2 mL of cell suspension into each well of the 6-well dish. Incubate the...anticipate that your viral stock will be higher titer, consider additional dilutions. Dilution Volume of Lentivirus...cells in each well. When calculating titer, only consider wells with less than 40% fluorescent cells. Titering...

  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...gels. They are shared through Addgene without licensing restrictions. Use these instructions to prepare... genome, many applications in biotech, and is considered the most studied Gram-positive bacterium, many...University of Minnesota recently deposited a comprehensive set of plasmids that can be used for either ...

  3. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically...Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great Tools for Gene...

  4. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...AAV Packaged on Request, an expansion of our viral vectors service, has officially launched! As of today... excited for all the ways this much-requested expansion will allow us to better serve our community. If...

  5. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single...further to provide multiplexed detection, higher sensitivity, and more. Read the paper in Science Find...

  6. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ... hard about picking a good mentor, and begin considering career options early. Tune in for great advice...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

  7. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...journals has risen steadily from 1995 to 2013. Considering citations to all articles, Anurag Acharya and...their own libraries and offering another way to consider and quantify the impact of scientific publications...

  8. Kit Free RNA Extraction

    Type
    Protocol
    ...tissues, extraction of RNA, precipitation, and resuspension. This protocol provides two options: using Solution...phase without disturbing the interphase layer, consider using a lower volume pipettor like a p200 to collect... freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of it and storing those...

  10. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...exemplifies our long-standing commitment to be responsive to the community’s needs and our commitment to...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every...

  12. Science Communication for Everyone

    Type
    Blog Post
    ... It is important to identify your audience and consider their background. Social media is a powerful tool...what you are trying to convey without having to extensively use Google search. Be careful about hidden connotations...

  13. Let There Be LITE Plasmids

    Type
    Blog Post
    ... another level with the addition of two light-sensitive ingredients - CRY2 and CIB1 – which they borrowed...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...

  14. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages...for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the...

  16. Editor's Choice, August 2016

    Type
    Blog Post
    ...CRISPR-based technique to generate C->T or G->A transitions in a target sequence in live cells. This technique...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

  19. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ... the spine. For binders of protocols and data, consider using color coded binders and tabs (for different...to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent storage...
Showing: 501 - 520 of 876 results