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We narrowed to 902 results for: Nes

Showing: 501 - 520 of 902 results

  1. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...culture of engagement in the issues at hand and eagerness to get, as well as give, information to make improvements...to the manager. Team members rarely rush to give honest feedback to the manager for a variety of reasons... frequency. Anonymity can be a great way to get honest feedback, but shouldn't be the only way.   From...

  3. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...perform pooled CRISPRi screens to identify essential genes and phage host factors, and identify sequence-specific...Knockout Library is a lentiviral library targeting 26 genes chosen from an analysis of human metastasis cohorts...knockout library targeting mouse tumor suppressor genes (TSG). Find the Mouse Metastasis CRISPR-Cpf1 Double...

  4. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ... more readily enable research. Often “normal” timelines that could be weeks to months were reduced to ...Institute                                       Chinese Academy of Medical Sciences University of Texas...Stanford University University of Iowa University of Minnesota See highlights from the COVID-19 collection Partnership...

  5. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ... cell’s surface proteins and this interaction determines the specificity and efficiency of the AAV serotype...each approach and to follow best practices and guidelines. It was wonderful to see the development of this...by the workshop participants  - a testament to openness and sharing in science.  We’re excited to support...

  6. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...Targeted manipulation Controlling stimulation Invasiveness  Choosing the best set of tools therefore depends...precise and the offset of stimulation is gradual. Invasiveness Both optogenetic and chemogenetic tools need...region Impacts all cells expressing receptors Invasiveness Mice require permanent intracranial implant ...

  7. An Addgene Summer Internship

    Type
    Blog Post
    ...able to obtain eight clones. We ran digested and undigested samples of the clones on an electrophoresis... and I were unsure if there were any successful clones because the bands were all in the wrong place. ...

  8. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same pattern...endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted...

  9. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...and can deliver large sequences and even entire genes. Figure 1: Mechanism of CAST systems. A Cas...systems; Cas12k in Type V-K systems; Cascade shown) combines with a donor template and transposon proteins ...Prime-Editing-Assisted Site-Specific Integrase Gene Editing), which combines prime editing with a site-specific recombinase...

  10. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...easy to spot meeting participants. They were the ones holding poster tubes (or as our baffled flight attendant...specifically in Dr. Zhang's introduction. His willingness to share reagents so freely with the academic...changing it from a puzzle to decrypt to a tool to be harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's...

  11. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...it takes to be a good scientist are not the same ones it takes to be a competent manager (much less a ...strong sense of engagement in the work Behind-the-scenes support from their bosses and employers. Availability...may not necessarily be comfortable with imposed deadlines (there’s that need for flexibility, sometimes ...

  12. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express...At Addgene, we have a vast collection of empty backbones that have been designed, tested, and published...vector). Check out our complete guide to empty backbones for additional details and guidance for choosing...

  13. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to...visualization or isolation, then using only a few lanes or a small portion of the gel.  What a waste?  I... pour over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less ...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...communication itself, not the communication method, that determines if something is scientific or science communication...defined by any number of characteristics. Common ones include age range, familiarity with the subject,...audience to engage if they so wish. (And let’s be honest: are you more likely to closely read an email sent...

  16. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...fall into one of two categories: ones induced by the researcher and ones that happen naturally.  Sample ...working with native proteins instead of denatured ones - well, don’t relax just yet. Proteins often change...

  17. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...sometimes impossible) timelines—others are usually waiting Work is concentrated to business hours (mostly), ...with peer mentors, to discuss your strengths, weaknesses, interests and passions is another great way ...

  18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...coat (and envelope), has a “gutted” genome with genes central to the replication process removed. This... the viral vector, increasing both safety and usefulness of the tool. In fact, most gutted genomes have...vectors to reproduce when experimentally necessary, genes for the required proteins are packaged in separate...

  19. What the HEK?

    Type
    Blog Post
    ... HEK is it good for?   As one of the older cell lines in the research community, many cellular research...organism. This pipeline is utilized to transduce cell lines for basic science research all the way up to the...HEK is wrong with it?   All cultured human cell lines are susceptible to contamination by mycoplasma and...

  20. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion ... be picked and sequenced for potential positive clones. Once you introduce your 3’-end “A” overhang insert...
Showing: 501 - 520 of 902 results