...optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal populations...how newly generated neurons integrate into the complex pre-existing circuit of the brain. Another study...anatomical regions of the brain receive inputs and compute outputs. In general, RABV monosynaptic tracing ...circuitry, and continue to contribute answers to the complex questions of how the brain operates. References...

...cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted expression was...retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in mouse, monkey...targeted cells in monkey and human retinas. By comparing the performance of promoters across these three..., Szabó, A., Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan...

...up clear tasks for each member of the team to accomplish so that many elements of the project were able...involved definitely contributed to our ability to complete the project on such a short timescale. Our article...extensive infrastructure of molecular and electronic companion technologies that has transformed the assay’s ...of Zika Virus Using Programmable Biomolecular Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids...

...protein capsid encapsulating the genome is majorly composed of three proteins that can be easily modified ...Delivering gRNA and Cas9 using adenoviral vectors As compared to other viral vectors, AdVs can provide a large...AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded...vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array...

...better at producing a compound of interest, thus allowing them to optimize the compound production process...fluorescent proteins have usurped old methods or complemented them in ways that make them much easier. A special...Although it is possible to make a timer system composed of two separate FPs (one that matures quickly ...

... capture large field-of-views (6). For more comprehensive reviews on several other LSFM configurations...multiplying couple-charged device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it...labeling in whole tissue (15).  CLARITY is also compatible with LSFM, a method termed COLM (clarity-optimized...spatiotemporal control over labeling methods as compared to transgenic animals (17).  These viral plasmids...

...For example, Drosophila lines with compound autosomes are completely infertile when mated to wild fruit...with appropriate measures. Barriers should be a component of all gene drive confinement strategies, but ...: I. Cage Studies of Chromosome Replacement by Compound Autosomes in Drosophila melanogaster. Genetics...

...his program. Initially, Provost made his labs completely open-ended, allowing every student to work on...Kowalski et al., 2016; Scott, 2021). In fact, completion of Provost’s CURE increased students’ confidence...them to near homogeneity using a 6XHis tag, and compared their specific activities by measuring NADH production...Poirier, V., Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell maintenance...

...this situation: You’ve spent hours scouring a company’s LinkedIn pages, given yourself the bathroom mirror...socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re sitting ...the candidate has what it takes to thrive at the company and add value, not just “do the job”. When they...these responsibilities. If you are in charge of completing many meticulous protocols or tasks, you’ve probably...

...gross comparison of AAV.PHP.eB and AAV.PHP.S to their parental vectors. To quantify this comparison, capsids....PHP.S had 1.5- to 2-fold higher GFP intensity compared to the strains they were derived from. They also...research. Visit Our AAV Guide Page for info on AAV components, common uses of AAV, and much more! Find ready-to-use...

... an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine...off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385...edits by ~45% and off-target A-to-I edies by ~94% compared to RESCUE.   Addressing the potential use of RESCUE... Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector...

...orders were affected by the COVID-19 pandemic, we compared the number of plasmid orders and new COVID-19 ...the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential...effects fairly well. However, we observed one complication in the China data – there is a period of 1 month... lockdown in China, then the observed data is compatible with an infinity-fold reduction in activity due...

...real time. When the PCR is complete, the results are analyzed by comparing the threshold fluorescence ...amplification reaction to take place.  When the PCR is complete, a droplet reader extracts the droplets from the...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...system, the possibility of creating a replication-competent virus via recombination between the transfer and...recombination events necessary to create a replication-competent virus. Second, Tat is eliminated through the addition...Lentiviral barcoding libraries have been used for complex applications like lineage tracing in heterogeneous...

...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money... few ways you can do this. If you are going to complete your colony PCR analysis in the same day, you ... product size on a gel   Now that your PCR is complete, it’s time to run the products on an agarose gel... larger sized products for positive clones (+) compared to negative clones. Finally, orientation-specific...

...is ideal for tracking large collections. Many companies, such as Thermo Fisher and Micronic, provide 2D... time. 1D barcodes can be purchased from many companies such as Corning. Tubes can even have specific ...specific barcodes etched or printed onto them by companies such as Tubewriter. Any lab can benefit from using...tubes themselves. Although we’ve developed our own computer system for tracking samples, it can be done easily...

...enChIP system enables us to express the CRISPR complex much easier in various cells, including primary...give to post-docs and graduate students as they complete their appointments in your lab?  Fujii: These ...survive as a researcher because of the tough competition for academic positions and poor grant situation...develop new technologies, hopefully they'll be completely different from what we are now doing in my lab...

...on OWW, including those for preparing your own competent cells. If you’re familiar with the process of ...service free of charge to scientists by allowing companies to pay publish their catalogs on the site. Like...of checking off each step as you painstakingly complete your experiment – one small victory in a long ...manage scientific data: Electronic lab notebooks compared with Google Docs and wikis - by Rory Macneil on...

... and the complexity of effectively deploying them in the lab somewhat mirrors the complexity of understanding.... Addgene’s Viral Vector Service Besides being complex to use, viral vectors are also difficult both to..., and jGCaMP8 viral preps distributed per year compared to other AAVs preps from 2016–2023. B) Inset showing...

...virus as self-complementary AAV (scAAV). However, scAAV vectors have limited size compared to single-strand...-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing, and ...virus’s most abundant proteins and a critical component for viral genome packaging. Addgene recommends...