We narrowed to 566 results for: PAC

1. Magnetic Control of Proteins: More than a Dream

   Type

   Blog Post

   ...-up studies. “We don’t have a vision of passive impact,” he says, noting that his team regularly shares...

2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   ... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

3. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   ...that are distant linearly but close together in space. The chromatin “picture” is then developed by detecting...

4. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   ...and not valued which will undoubtedly negatively impact your work and your life. It’s up to you to decide...

5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   ...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

6. Plasmids 101: Optimizing Plasmid Yields

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   Blog Post

   ...strain Differences between E. coli host strains can impact plasmid yield. Check the bacterial strain to see...

7. Experimenting with New Careers while in Grad School

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   Blog Post

   ... once a week to get recharged. The fun change of pace helped break up the negativity and allowed me to...

8. Genome-wide Screening Using CRISPR

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   Blog Post

   ...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...

9. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   ...wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs are fluorescent when ectopically...

10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future of the...

11. Neurodegeneration Research Collection

    Type

    Collection

    ...Community (Link opens in a new window) Research workspace on Protocols.io (Link opens in a new window) ....

12. Great Results Start with Great Standard Curves

    Type

    Blog Post

    ... be using ahead of time to see what factors may impact the dynamic range of the assay. Try to minimize...

13. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ...gene function while using fewer sgRNAs. These new compact libraries will allow scientists to conduct genome-wide...

14. DNA Purification Without a Kit

    Type

    Blog Post

    ...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...

15. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    ... is the transferable skills that create a full package and add an extra shine to your work. Knowing your...

16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ...affinity for endogenous molecular targets such as paclitaxel. Unlike other systems, no genetically encoded...

17. CRISPR Antimicrobials

    Type

    Blog Post

    ...containing a phage origin of replication that can be packaged into replication-incompetent phage particles using...

18. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    ...decrease. Furthermore, the size of the parts also impacts ligation efficiency, as concentration does not ...

19. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...

20. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    ...candidacy, beyond what is on your resume. It is a space for you to explain how your skills and experience...