We narrowed to 783 results for: SCR;

1. Plasmids 101: TOPO Cloning

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   Blog Post

   ...cassette allowing a researcher to perform blue-white screening after transformation - self joining of the vector...

2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   Blog Post

   ...must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can ...

3. Plasmid Cloning by PCR

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   Blog Post

   ...the downstream steps. For this example, we will describe how to copy a cDNA from one vector into a new ...

4. Don’t Wait To Launch Your Health Science Startup

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   Blog Post

   ... own experiences founding and running miLab to describe some key lessons I learned while becoming a health...

5. Extracurricular Activities for a Strong Science Career Path

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   Blog Post

   ...you particularly good at writing and editing manuscripts? Are you interested in scientific policy? Next...

6. K. phaffii: Rising to the Occasion

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   Blog Post

   ...Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid System Additional...

7. Scientists Map the SARS-CoV-2-Human Interaction Network

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   Blog Post

   ...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction...

8. 25 Work From Home Tips for Scientists

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   Blog Post

   ...visualizing your data, such as R or Python. Write a manuscript. If you have all the data you need to begin writing...

9. What Good Citizenship Can Do for Reproducibility in Science

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   Blog Post

   ... LMB in Cambridge UK and then a postdoc at The Scripps Research Institute in La Jolla, California. She...

10. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    ...site-saturation mutagenesis, and medium-throughput screening, she increased the affinity of the sensor for ...

11. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post

    ...producer cells and purification protocols often describe a process that begins with cell lysis to release...

12. Creating accessible biology activities in schools with BioBits

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    Blog Post

    ...produce that green fluorescent protein through transcription and translation. Unfortunately, having live ...

13. Learning Technical Skills at a Lab Central Workshop

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    Blog Post

    ...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

14. Visualizing Translation at the Single Molecule Level

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    Blog Post

    ...cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent...

15. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post

    ...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

16. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post

    ...all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using...

17. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post

    ...relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris Richardson...

18. How to Lead a Great Meeting

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    Blog Post

    ...points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving...

19. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post

    ...has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard...

20. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post

    ...time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction...