We narrowed to 570 results for: SHA

1. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   ...transparent about his own biases--many of which will be shared by fellow scientists--but that doesn’t make his...

2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   ...A few months ago, we shared an introduction to immunofluorescence (IF) - a common method for visualizing...

3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   Blog Post

   ... order of 700 terabytes per gram—and form the X-shaped chromosomes we all know and love. To understand...

4. Lentiviral Vector Uses and Overview

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   Blog Post

   ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...

5. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   ...References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J. Beta-galactosidase gene fusions for...

6. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   ...samples are easy to locate in freezers that may be shared with multiple people or even multiple labs. Careful...

7. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   ...if you throw in some passion for our mission to share reagents with scientists around the globe, the end...

8. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   ...Demand for grant professionals Why have we seen a sharp rise in professional development targeted to the...

9. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   Blog Post

   ...streamline your plasmid extractions. Use this protocol shared by Dr. Rob Hurt for high-throughput minipreps in...

10. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    ...briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based: if ...

11. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    ...Jain and Srivastava 2013). They are also easily-shareable tools within the scientific community, as they...

12. Viral Production at Addgene

    Type

    Blog Post

    ...references Resources at the Addgene blog Find and share AAV Data with Addgene's Data Hub Digital Droplet...

13. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...depositing lab whenever available. Addgene also shares the protocols we’ve developed for the libraries...

14. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post

    ..., et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute ...

15. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ... out this interview with the Nicola Patron, who shared the MoClo plant kit via Addgene. For us, transient...

16. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...a powerful method to dissect the individual and shared contributions of transcript isoforms to neuronal...

17. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    ...location, with more space to grow and help scientists share resources in new ways. Along with a new location...

18. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ...whiteboard. Thank everyone for the opportunity to share your research vision with them. Do not noticeably...

19. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    ...and loxP sites differ at the nucleotide level but share an overall structure of 13 base pair palindromic...

20. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    ...consider. Agarose beads are sponge-like and vary in shape and structure. Their porous surface provides a large...