We narrowed to 581 results for: SUB

1. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   ...correct for this, compensation has to be applied to “subtract” this spillover and correctly distinguish between...

2. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   ...et al., 2024) have highlighted how chromatin substantially affects prime editing outcomes across many genomic...

3. Pooled Library Amplifications

   Type

   Blog Post

   ...Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 20 bp ...

4. PhD Applications After COVID

   Type

   Blog Post

   ...that you reach out to potential advisors before submitting your application. I found this step to be incredibly...

5. Academic vs. Industry Postdocs

   Type

   Blog Post

   ...will be reviewed by an internal committee before submission to ensure that it does not divulge any propriety...

6. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   .... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

7. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   ... anneal incorrectly, MMEJ may also introduce, substitute, or delete nucleotides in addition to the expected...

8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   ...would work for your system. Addgene has depositor submitted protocols and links to a CRISPR forum where you...

9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   ...-coding sequence using a recoding strategy; a subsequent drive can restore the wild-type sequence (save...

10. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    ...experiments. Fluorescence is the emission of light by a substance that has absorbed light. The emitted light is ...

11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...incredible is the bioeconomy is already pretty substantial. It's estimated that genetic engineering as applied...

12. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    ... global activity in our particular scientific subfields.  Plasmid requests correlate with paper publication...

13. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    ... from Addgene and the CLOVER Center, each one submitted by researchers like you. Reports are reviewed ...

14. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    ...kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications you...

15. Tips for arabidopsis transformation

    Type

    Blog Post

    ...likely that such a construct will be silenced in subsequent generations. You will know if you obtained a ...

16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    ...PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about other CRISPR ...

17. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    ...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...

18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...AAV as a tool for gene expression in mammals. Subscribe to Viral Vector Blog Posts Additional Resources...

19. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...use, it’s worth scheduling the second and other subsequent meetings. The number of participants, breadth...

20. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    ...These pages provide helpful information about the subject, link to related guides and protocols, and provide...