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...biochemistry department at the University of Massachusetts Medical School. We are working with postdocs...to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary...

...COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from Ando et al. 2023, under CC-BY...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...

...carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use...Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher titer...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...excision or insertion, inversion, translocation or cassette exchange. By utilizing recombinases to manipulate...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... hybrid gRNA library deletes human alternative cassette exons and disrupts genes with alternative exons...

... website so scientists can view public circRNA datasets and download the custom python scripts needed ...phage vectors with CRISPR/cas9 sensitizing gene cassettes. Thse vectors are engineered to replace the drug...

...while lentivirus requires a BSL-2 setup. This requires a less involved setup when working with mice and slightly...

...different names depending on your flow cytometer’s setup. For instance, BV510 and AmCyan have almost identical...practice, you may be able to do your compensation setup on your experimental samples without needing to ...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines are...

... used to compare between different experimental setups. A colocalization analysis example is given in ...detection of one fluorescent signal in a channel setup for a different fluorescent signal) and photobleaching...

...). Misek and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic...by bias within the design of the experiment or dataset itself. It is important for scientists to not only...

...incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ...doi.org/10.1016/0378-1119(88)90117-5  Lale, R., Brautaset, T., & Valla, S. (2011). Broad-host-range plasmid...

...doi.org/10.1006/mthe.2000.0116 Gutierrez-Guerrero A, Cosset F-L, Verhoeyen E (2020) Lentiviral Vector Pseudotypes...Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L (2002) Lentiviral vectors pseudotyped with ...

...GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across 8,792 organisms...biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...

..., and are therefore able to infect only a small subset of the cells used in a given experiment. For this...Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports 20.3 (2017...

...complement each other to restore the full-expression cassette. AAV’s natural ability to reassemble fragmented...2017) CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756...

..., we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest...kb or less. Luckily, most of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc...

... Its Fruit Is Sweet Time If you require a quick onset of expression, AAV may not be the best tool to use...delivery indicates ~7-21 days are required for the onset of gene expression. However, it has yet to be determined...

...gene of interest with an antibiotic resistance cassette.   Substrate DNA Design and Generation Whether...expression of the recombineering system.   This setup can be also be used to modify chromosomal genes....

... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP...