Skip to main content

We narrowed to 738 results for: Sis

Showing: 501 - 520 of 738 results
  1. Summer Fun at Addgene!

    Type
    Blog Post
    ...tackling complex questions, and giving tips for consistently generating solid data. If you’re ready for the...
  2. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and ...purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the following solutions...phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom phase...
  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...
  4. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...scientific literature.  -In light of the current Ebola crisis, Science and Science Translational Medicine have...
  5. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer , to...
Showing: 501 - 520 of 738 results