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We narrowed to 731 results for: Sis;

Showing: 501 - 520 of 731 results
  1. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...
  2. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...scientific literature.  -In light of the current Ebola crisis, Science and Science Translational Medicine have...
  3. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer , to...
  4. Let There Be LITE Plasmids

    Type
    Blog Post
    ...ingredients - CRY2 and CIB1 – which they borrowed from Arabidopsis thaliana. The results are light-inducible transcriptional...
  5. Kit Free RNA Extraction

    Type
    Protocol
    ...general steps of this protocol include homogenization/lysis of cells or tissues, extraction of RNA, precipitation...isolation will depend on how effective your cell lysis protocol is. While simple homogenization is effective...
Showing: 501 - 520 of 731 results