We narrowed to 562 results for: Spr

1. Viral Vectors 101: The Retroviral Lifecycle

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   Blog Post

   ...Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13...

2. A Guide to Starting Your Own Journal Club

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   Blog Post

   ... a week for several months at a time during the spring and fall semesters. Our scheduling was in part ...

3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...individual molecules. Poor photon budgets are a widespread issue with fluorescent proteins—even the thriftiest...

4. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...strand RNA viruses: The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166...

5. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   ...done easily with a barcode reader and an Excel spreadsheet. Start by scanning the sample barcodes into the...

6. The Importance of a Fun Workplace: Company Culture at Addgene

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   Blog Post

   ...something fun is going on and to get there fast! Spread word of your plans by email, gong, evite, posters...

7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   Blog Post

   ... in the wildtype protein and facilitating its widespread use in research. Many other mutations have since...

8. Save Time with Transient Plant Leaf Transformations

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   ...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...

9. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain." Nature biotechnology...

10. Neuronal labeling with Spaghetti Monster

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    Blog Post

    ...fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009): pdb-top63. PubMed...

11. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc...

12. Antibodies 101: Reading a Flow Plot

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    Blog Post

    ... density of cells, while contour lines that are spread apart show a low density of cells (Figure 7).  ...

13. Getting the Most from Your Lentiviral Transduction

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    ...Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org...

14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ... genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over...

15. Important Considerations in Optogenetics Behavioral Experiments

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    ...collaboration and the exchange of ideas. The rapid spread of optogenetics throughout the neuroscience field...

16. Plasmids 101: Control Plasmids

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    ...or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate as they...

17. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ... phosphoglycerate kinase gene.  Constitutive  Widespread expression, but may vary by cell type. Tends ...

18. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous...

19. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson...

20. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

    ...genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed...