We narrowed to 617 results for: TIL;

1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   Blog Post

   ...GFP, our most-requested GFP(1–10) and GFP(11) Versatile protein tagging in cells with split fluorescent...

2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   Blog Post

   ...you can make big changes in your career path and still find comfort and happiness there. Read all of the...

3. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...targeting the same cell class in all three species are still greater than random chance. The chances of a promoter...

4. Design Tips for Prime Editing

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   ...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different...

5. Hot Plasmids February 2024

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   ...acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a recombinant...

6. Adapting Toehold Switches to Detect Zika Virus

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   Blog Post

   ...role in helping to monitor and slow this spread until vaccine programs can be put in place to provide ...

7. Tips for Titering Your Lentiviral Preps

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   ...Fluoresence Titering Protocol Another popular approach utilizes the antibiotic resistance genes carried on transfer...

8. Gaining Leadership Skills Volunteering at a Professional Organization

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   Blog Post

   ...invaluable experience and even after 5 years, was still improving my skills. My involvement with the NPA...

9. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...capabilities. So don’t throw old equipment away until you ask your local makerspace if they want to hack...

10. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    ... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...

11. PhD Applications After COVID

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    ...canceled, taking advantage of virtual meetings can still be a great opportunity to introduce yourself and...

12. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    .... If you don’t have access to a list of posters until the day-of, take a moment to step aside to read ...

13. Plasmids 101: Protein Expression

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    Blog Post

    ...DNA constructs designed for mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust...

14. Polymerase Chain Reaction Overview and Applications

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    Blog Post

    ...saving both time and effort. Scientists can also utilize multiplex qPCR to quantify the concentrations of...

15. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...myo-inositol oxygenase), providing enzyme protection while still allowing substrates and products to diffuse freely...

16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...along for the ride throughout all of these steps, until its connection with DNA is broken during cross-link...

17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    ... References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning System for Characterization...

18. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    ...wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to ...

19. Academic vs. Industry Postdocs

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    Blog Post

    ...that they were having a positive experience, I was still incredibly nervous about leaving academia. A lot...

20. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...because of the second passage and because you wait until the majority of cells have died to collect your ...