.... In vivo, these recombination reactions are facilitated by the recombination of attachment sites from...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry vector.... to 4 DNA fragments, in a specific order and orientation, in one tube, into one Gateway vector to produce...

...inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  This process...For example, multiple studies show that certain mutations of the BAP1 gene play a role in susceptibility...individuals, asbestos has not been banned in the United States and many other countries, and it is still imported...

...at the same time increasing interdisciplinary citations in their own work. Of course not all research ...ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their contribution...Archambault. "The decline in the concentration of citations, 1900–2007." Journal of the American Society for...

...maintaining their friendships. I put “neutral” in quotations here because I would not have considered myself...handy: Empathy: If you often find yourself in confrontational situations with others because you simply cannot...competing, or accommodating, if you find yourself gravitating most frequently towards these approaches, you...

...training and experience, better align trainee expectations with job outcomes, and increase diversity in...need to know in order to even assess the current state of research became more clear throughout the various...information publicly available may realign trainee expectations with actual job outcomes and/or disincentivize...

...research is dependent on a pathology microtome/cryostat, then making a careful decision on the condition...for suggestions to meet each others’ needs and limitations. Everything is negotiable. Try asking for discounts...research, then it’s worth working within their limitations. 3. Always provide value to others This is a ...

...galactose3 AAV receptor2 Laminin receptor (LR)3 Putative integrin3 1Wu, Miller, et al., 20062Pillay...for mice in vivo. To target... Try using... Citation CNS AAV1, AAV2, AAV5, AAV8, AAV9, AAVrg Aschauer.... (2006). Α2,3 and α2,6 N-Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated...

...across the genome) has been implicated in disease states, and targeting the epigenome may make certain processes... spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves methylation kinetics such that cytosine...transduction. Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9...

...have intricacies that aren’t always mentioned in static written protocols. It is therefore a bit ridiculous...experiments with too few replicates leading to suspect statistical significance (you can read more about this problem...experimental design practices including talking to statisticians when necessary and encouraging interlab peer...

...of hands-on lab work for a dissertation. I chose to carry out my dissertation research in a lab in California...outcome for a specific combination of genetic mutations. This is a particular challenge in pancreatic ...

... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ...work in certain applications — the most common limitation is that certain probes are not very good at crossing...

...biochemist, a geneticist, a microscopist, a biostatistician, and that one golden-handed technician who ...Andrew, the team’s founder, to create a mission statement in the first few meetings and we’ve volunteered...like kudzu. Think of a soccer player who gets a reputation for taking risky shots at the goal rather than...

...Kishor Jadhav. Mycoplasmas can remain in the dry state for a longer time. When they come in contact with... is required in this process because result interpretation might be difficult. The low density of mycoplasmas...by these bacteria can hinder correct result interpretation. Also, the signals from the nuclear area make...

...Location, location, location! What do buying real estate and using AAVs have in common? The three most important... location, location! Just like this common real estate mantra, the choice of serotype (which determines...identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory connections (3). These facts beg the question...

... to assemble several DNA parts in a specific orientation using Type IIS restriction enzymes (Weber et ... researchers to expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified...acssynbio.3c00423. Pérez-González, Ana, et al. “Adaptation of the GoldenBraid Modular Cloning System and...

...Although it was a huge innovation, there was a limitation to this approach. CBEs and ABEs are base transition...repair (BER), the cellular process that catches mutated U’s in DNA and ideally converts them back to Cs... Cas9s that recognize different PAMs. Their computational tool for predicting CGBE editing outcomes and...

...previously-designed tyr gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic...recombineering, with 96-99% efficiency for point mutations and 66% efficiency for small insertions. Multiplexing...degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are delivered on a different...

... had the lowest background activity in the dark state and highest fold induction at 31X. By using a slit...by blue light (470nm), paCas9-1 induced indel mutations via NHEJ (frequency of 20.5%) and induced modifications...significantly alter the system’s efficiency at generating mutations when activated with light and lowered background...

...sequence cells to verify excision didn’t introduce mutations Adenovirus Non-integrating Low Medium Generates...and/or oncogenes, potentially leading to the presentation of a cancer-like phenotype in infected cells...stringency test for mouse iPSCs, tetraploid complementation, involves injecting tetraploid blastocysts ...

...currently at 4,651 as of April 8, 2021) Find these stats and more on the addgene.org homepage! You can track...of plasmids right below the search bar and more stats further down the page....