We narrowed to 798 results for: Tec

1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

   Type

   Blog Post

   ...Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted...activity. While they serve similar purposes, both techniques offer researchers different advantages and limitations...

2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

   Type

   Blog Post

   ...overcomes all of these labor-intensive and invasive techniques to reliably label any post-mitotic cells in Drosophila...et al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across cell types...

3. Networking and Inspiring Young Scientists at Freshers Fairs

   Type

   Blog Post

   ...learn about Synthetic Biology and exciting new technologies in the field. One of the main aims of the society...networking to promote the creation of start ups and biotech spin offs. The Synthetic Biology Society also aims...

4. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

   Type

   Blog Post

   ...field. Advances in genome engineering & CRISPR technology That afternoon's workshop, entitled "Advances...amazement at how much has been done with CRISPR technology in just 2 years and excitement about how much...

5. The Strength of Story Telling

   Type

   Blog Post

   ...STAR (Situation, Task, Action, Result) interview technique, which has been addressed in much better pieces...story should be too. Use drama Use any rhetorical technique you have in your arsenal to make sure people pay...

6. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

   Type

   Blog Post

   ...Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward the red...However, a challenge in optimizing base editing technologies across several cell types is the lack of a rapid...

7. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   ... genetically encoded antibody based probe that detects the widely used HA epitope in diverse cellular ...dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and scaffolds...

8. Single-cell tracking of lineage and identity with CellTag

   Type

   Blog Post

   ...desired identity. The development of single cell technologies, such as single cell RNA-seq (scRNA-seq), now...multi-generational cell lineage trees. CellTags were detected over a 10-week period. Their expression decreased...

9. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

   Type

   Blog Post

   ...postdoc association, started a speaker series or biotech club, managed their own blog or podcast, initiated...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for...

10. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type

    Blog Post

    ...scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that ...applied for the government sponsored Paycheck Protection Program loan and received enough funds to make...

11. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    ...The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833...

12. Antibodies 101: Chimeric Antibodies

    Type

    Blog Post

    ...secondary to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B...

13. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    ...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to... The development of monoclonal antibody (mAb) technology circumvented many of these issues by creating...

14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type

    Blog Post

    ...bacterial strains, such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI...Kamens J, Fan M (2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

15. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    ...containing lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid...measurements, or monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin...

16. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type

    Blog Post

    ...reliably confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an...minutes and the scripts  can be easily customized to detect other sequences of interest.   While the VGS process...

17. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    ... to detect mutants, but this method limits screening to a few hundred worms, making the detection of rare...

18. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    ...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and...

19. Extracurricular Activities for a Strong Science Career Path

    Type

    Blog Post

    ...translational approaches for scientific tool and technique development. However, careers outside of the lab...Management Leadership Networking University technology transfer office positions   Market analyses...

20. The Importance of High Titer for AAV Transductions

    Type

    Blog Post

    ...viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production...