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  1. Management for Scientists: What Makes a Good Manager Anyway?

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    Blog Post
    ...recognition come from the successes of the team. A strong sense of engagement in the work Behind-the-scenes...    This is the first in a 5 part series on Introduction to Managing People for Scientists. Subscribe...follow the entire series as it is published. Good introductory reference books on managing people: Skills ...
  2. Choosing Your Perfect Empty Backbone

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    Blog Post
    ...function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first...expression system and go for a lentiviral or a retroviral backbone. This is considered to be a more robust...great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression...
  3. CRISPR/Cas9 FAQs Answered!

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    Blog Post
    ...Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate these FAQs, Le Cong worked...gRNAs with the Cas9 nickase which will together introduce cleavage of the target site with efficiency similar...using CRISPR-Cas9 at the lab bench Q4: After the introduction of a mutation into the genome, how can cells...
  4. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ... store a record of invading phages but to also destroy the phage upon re-exposure (2, 3). More specifically...complex binds to the foreign DNA, a cut is made to destroy the invader. Wikipedia, accessed 25 November...ultimate victor will be the species who has the strongest will to survive. Genome engineering and future...
  5. Plasmids 101: Origin of Replication

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    Blog Post
    ...the origin of replication (ori) and all of its control elements. The ori is the place where DNA replication...depending on how they are regulated. Generally, control of replication is referred to as "relaxed" or "...Vectors Copy Number+ ori Incompatibility Group Control pUC ~500-700 pMB1 (derivative) A Relaxed pBR322...
  6. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    Blog Post
    ...One of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists...Gene Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including...number of AAV preparations this level of quality control is sufficient but for viral vector core facilities...
  7. Summer SciComm Series: Master of SciComm

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    Blog Post
    ...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...address this obstacle in its many forms. A short stroll from my office to the tearoom would often illustrate...Cognitive LoadSummer SciComm Series: Modes of CommunicationIntro to Our Summer SciComm Series!...
  8. Antibodies 101: Epitope Availability

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    Blog Post
    ...cases, pay special attention to designing your controls in validation, as you may need to test different...great deal of frustration when designing and troubleshooting immunoimaging assays. It can also elucidate...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...
  9. Another Pathway into Cells: iTOP

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    Blog Post
    ...problems. For instance, it’s much more difficult to introduce a gene of interest into primary cells, so most...both purified CPP-tagged protein and an untagged control could enter cultured cells and activate expression...reporters Previous Protein Delivery Techniques: Introduction of macromolecules into cultured mammalian cells...
  10. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s axon. While...even larger area, thus necessitating even higher retrograde AAV titers for successful transduction. In another...from and what do they mean? AAV Vector Quality Control: Going the Extra Mile with NGS AAV:  Versatile ...
  11. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post
    ...transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the conventional AAVs...target less accessible cells, such as brain tumor stromal cells in a mouse model of glioblastoma (Volak et...vector-mediated genetic modification of brain tumor stromal cells after intravenous delivery." Journal of neuro-oncology...
  12. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post
    ...reproduce someone else’s result. Then, they hit trouble. The experiment won’t work. Even if it does, they...to share with other researchers to help them troubleshoot and understand. All of this communication and... of incentives. Right now, researchers are not strongly rewarded for being transparent in their communication...
  13. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    Blog Post
    ...independently verified, the watching world erupted in controversy. Briefly, there are two overlapping, valid critiques...within their respective mandates, to tackle a controversial and ethically fraught rapidly emerging technology...good research practice: computer simulations, in vitro research, animal models, and several stages of human...
  14. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...components, it may be useful to describe the means of introducing genetic material (such as plasmids) into mammalian... comparable to bacterial transformation (the introduction of DNA into bacterial cells); however, the techniques...plasmid to be expressed transiently. Since the DNA introduced in the transfection process is not integrated...
  15. Plasmids 101: Inducible Promoters

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    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...promoters can be regulated by positive or negative control. Positive inducible In the OFF state, the promoter...system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally inactive and cannot...
  16. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post
    ...acid in the media and the tRNAs and synthetase introduced to the bacteria using plasmids. Rapid expansion...plasmid. Third, a second plasmid will be needed to introduce the gene of interest containing the chosen codon...researchers, from selective protein ligation through introducing ncAAs with protein modifications of interest ...
  17. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ... by stories. In retrospect, I should have expected this given that the brain controls everything we do... at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse Optogenetics Plasmids ...
  18. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post
    ...growth rates will be higher in treatment versus control, and you think there are four important times to...preregistration, but usually with a fully fleshed out introduction section) to one of 222 participating journals..., they will place special emphasis on positive controls and good sample sizes, concerns that are too easy...
Showing: 501 - 520 of 914 results