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  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies widely with gRNA sequence. Both groups used multiple...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

  2. Plasmids 101: FLEx Vectors

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    Blog Post
    ..., FLEx is currently being used to study a wide variety of in vivo conditional gene rescue experiments,...switches to conditionally test the function of various point mutations and truncations in the absence ...

  3. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...share plasmids, especially those of the CRISPR variety (see slides from my talk below). Largely organized...identify. There were dozens of different mushroom varieties alone. At least I could tell that they were mushrooms...

  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...have been discovered to play a large role in a variety of diseases, ranging from cancer, to diabetes, ...transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015): 533-537. ...

  6. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...recommend the use of an electronic pipettor with variable spacing (such as the Viaflo from Integra Biosciences... clones. The technique is applicable to a wide variety of cell lines, from HEK to CHO cells to neural ...

  8. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...you want to be!  References Becoming the boss. Harvard Business Review, January 2007. Three Rules for ...

  9. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...human disease. However, the rate of transfer was variable. They measured transfer rates by comparing the... of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to...

  11. Hot Plasmids: Winter 2025

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    Blog Post
    ...improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings of spontaneous...assay dozens of GPCRs across many cell types in varying conditions, revealing context-dependent responses...

  12. Common Injection Routes in Mice

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    Blog Post
    ...factors. You have to weigh the pros and cons of various methods and choose what is best for you — and the...array of purposes, but mainly for the delivery of various therapies and viral vectors. This injection sounds...

  13. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ...are Sf9 cells, which are derived from the pupa ovarian tissue of fall armyworms (Spodoptera frugiperda...protein production. Titration of baculovirus can vary depending on the protocol used. Plaque assays are...

  14. Plasmids 101: Degron Tags

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    Blog Post
    ...destabilizing domains which will be identified by various enzymes and sent to the proteasome for degradation...protein levels aren’t limited to ‘on’ and ‘off’; varied levels of drug concentration can yield intermediate...

  15. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...viral genome in eukaryotic cells.1,2 Since then, a variety of IRES sequences have been discovered - many from... reporter gene). Addgene's collection offers a variety of plasmids to express two or more genes, some ...

  16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...generating single or multi-gene knockouts in a wide variety of cell types, gene editing using homology directed...performing CRISPR experiments using RNPs is the variety of methods that can be used to deliver a Cas9-gRNA...

  17. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’t “broken,” their...that nanobodies come in 1x and/or a 3x cysteine varieties. This refers to the number of cysteine sites the...

  18. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...we just made the sample bigger? In ExM, and its various derivatives, biological samples are enmeshed in...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...
Showing: 501 - 520 of 767 results