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We narrowed to 766 results for: Vars

Showing: 501 - 520 of 766 results
  1. Getting to Know Expansion Microscopy

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    Blog Post
    ...we just made the sample bigger? In ExM, and its various derivatives, biological samples are enmeshed in...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...
  2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post
    ...generating single or multi-gene knockouts in a wide variety of cell types, gene editing using homology directed...performing CRISPR experiments using RNPs is the variety of methods that can be used to deliver a Cas9-gRNA...
  3. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post
    ... antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’t “broken,” their...that nanobodies come in 1x and/or a 3x cysteine varieties. This refers to the number of cysteine sites the...
  4. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post
    ...SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies widely with gRNA sequence. Both groups used multiple...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...
  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...within each color also exist with only slight variations separating them.  Please note that many fluorescent...fluorescent biosensors has been designed to detect a variety of intracellular conditions, including ion (such...
  6. Your Lentiviral Plasmid FAQs Answered

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    Blog Post
    ...collection of lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated...breaks down the biosafety concerns associated with various aspects of lentiviral transduction along with suggestions...
  7. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression, the codons that encode a protein...3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base pair. ...
  8. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post
    ...Since the efficiency of CRISPR/Cas9 genome editing varies across targets, they tested two genes in mice. ...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...
  9. Using Video to Share Your Science: We Share Science

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    Blog Post
    ... able to quickly synthesize information from a variety of fields in order to provide useful solutions ...been a long brewing frustration for me. I read a variety of psychology, business, personnel, and sociology...
  10. 5 Reasons to Use Reddit for Science Communication

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    Blog Post
    ... participate in forum discussions about a wide variety of topics. Individual forums are hosted on subreddits...sometimes, civil. r/Science in particular hosts a variety of conversations with people who work in many different...
  11. CRISPR Activation: A Practical Guide

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    Blog Post
    ... Cas9 activator should I use? There are a wide variety of activators you can use for your experiments....Addgene Blog Learn how to Choose the Best Cas9 Variant for Your Next Experiment Find out More about Genome-wide...
  12. Hot Plasmids: Fall 2024

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    Blog Post
    ...IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Tension Sensor (Zhong et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino...
  13. CRISPR-mediated Plant Base Editors

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    Blog Post
    ...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID...
  14. Transferable Skills Guide: Career Planning Resources

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    Blog Post
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...indeed.com or monster.com, where you’ll find a variety of listings. Post your CV and make it visible, ...
  15. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...save researchers significant time and removes a variable from the lengthy process of validating and using... (Figure 4), our catalog includes vectors in a variety of serotypes, including the retrograde AAVrg and...
Showing: 501 - 520 of 766 results