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We narrowed to 772 results for: Vars;

Showing: 501 - 520 of 772 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...destabilizing domains which will be identified by various enzymes and sent to the proteasome for degradation...protein levels aren’t limited to ‘on’ and ‘off’; varied levels of drug concentration can yield intermediate...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...are Sf9 cells, which are derived from the pupa ovarian tissue of fall armyworms (Spodoptera frugiperda...protein production. Titration of baculovirus can vary depending on the protocol used. Plaque assays are...

  3. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...you want to be!  References Becoming the boss. Harvard Business Review, January 2007. Three Rules for ...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...recommend the use of an electronic pipettor with variable spacing (such as the Viaflo from Integra Biosciences... clones. The technique is applicable to a wide variety of cell lines, from HEK to CHO cells to neural ...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...viral genome in eukaryotic cells.1,2 Since then, a variety of IRES sequences have been discovered - many from... reporter gene). Addgene's collection offers a variety of plasmids to express two or more genes, some ...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...we just made the sample bigger? In ExM, and its various derivatives, biological samples are enmeshed in...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...generating single or multi-gene knockouts in a wide variety of cell types, gene editing using homology directed...performing CRISPR experiments using RNPs is the variety of methods that can be used to deliver a Cas9-gRNA...

  10. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’t “broken,” their...that nanobodies come in 1x and/or a 3x cysteine varieties. This refers to the number of cysteine sites the...

  12. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies widely with gRNA sequence. Both groups used multiple...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

  14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...within each color also exist with only slight variations separating them.  Please note that many fluorescent...fluorescent biosensors has been designed to detect a variety of intracellular conditions, including ion (such...

  15. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...collection of lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated...breaks down the biosafety concerns associated with various aspects of lentiviral transduction along with suggestions...

  16. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression, the codons that encode a protein...3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base pair. ...

  19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Since the efficiency of CRISPR/Cas9 genome editing varies across targets, they tested two genes in mice. ...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...
Showing: 501 - 520 of 772 results