Plasmids 101: Knockout/Knock-In Plasmids
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Blog Post
...researchers often use the Cre/Lox system described earlier. In this case, you design your targeting vector...
We narrowed to 599 results for: arl
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Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...plasmid has been requested by labs around the globe nearly 400 times. Researchers have been excitedly testing... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post... might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...increasing the signal from the second FP. Several early FRET biosensors were made with CFP and YFP, cyan... -
Experimenting with New Careers while in Grad School
TypeBlog Post...Indiana University School of Medicine. She is particularly interested in science communication and fundamental... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post... TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance ... -
Genome-wide Screening Using CRISPR
TypeBlog Post...cell types that are of low abundance are not particularly well suited for genome-wide screening. Avoid... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post...www.protocol-online.org/ Protocol Online is one of the early protocol sharing websites. It was created in 1999... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...many efforts in open science, Endy was recognized earlier this year by the White House as a Champion of Change... -
Tips for arabidopsis transformation
TypeBlog Post...transformant. Use young plants: If you transform early, your plants will still have plenty of time to produce... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...transfection (B in the figure above), are in the early stages of development. David Liu’s Lab has demonstrated... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ... -
MXS Chaining
TypeBlog Post...robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental needs.... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...cargos must be flanked by the L and R ends - thus, scarless insertions are not currently possible, unlike ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post.... Most notably, China was affected by COVID-19 earlier than the rest of the world, from mid-January to... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...adventure, just remember that even though these early steps don’t even involve antibodies, they are still... -
Getting to Know Expansion Microscopy
TypeBlog Post...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...