We narrowed to 581 results for: arl

1. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   ...Indiana University School of Medicine. She is particularly interested in science communication and fundamental...

2. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   ... TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance ...

3. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   ...cell types that are of low abundance are not particularly well suited for genome-wide screening. Avoid...

4. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   ...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...

5. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   ...COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition...

6. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...many efforts in open science, Endy was recognized earlier this year by the White House as a Champion of Change...

7. Great Results Start with Great Standard Curves

   Type

   Blog Post

   ...consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use duplicates or...

8. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   ..., Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor...

9. Getting to Know Expansion Microscopy

   Type

   Blog Post

   ...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...

10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    .... Most notably, China was affected by COVID-19 earlier than the rest of the world, from mid-January to...

11. DNA Purification Without a Kit

    Type

    Blog Post

    ...extraction method described in Kim and Morrison is nearly identical to that of commercially available kits...

12. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    ...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...

13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    ...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from...

14. Tips for arabidopsis transformation

    Type

    Blog Post

    ...transformant. Use young plants: If you transform early, your plants will still have plenty of time to produce...

15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    ...cargos must be flanked by the L and R ends - thus, scarless insertions are not currently possible, unlike ...

16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    ...adventure, just remember that even though these early steps don’t even involve antibodies, they are still...

17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    ...transfection (B in the figure above), are in the early stages of development. David Liu’s Lab has demonstrated...

18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

19. MXS Chaining

    Type

    Blog Post

    ...robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental needs....

20. Plasmids 101: Colony PCR

    Type

    Blog Post

    ...  PCR set-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction:...