We narrowed to 648 results for: ato

1. Addgene's Tips for Plasmid Quality Control

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   ... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...

2. Plasmids 101: Golden Gate Cloning

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   ...for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct order in...

3. Career Coaching for Scientists: Why and Where Do I Find One?

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   ...help you. A good coach usually will be a clear communicator and make direct suggestions for improvement ...

4. 5 Steps to Writing Grant Proposals for Grad Students

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   ...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...

5. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   ... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...

6. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...general consideration for behavioral testing in a laboratory, a high level of experimental control is required...

7. Plasmids 101: The Wide World of Natural Plasmids

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   ...Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer...

8. Making CRISPR Plasmids Using Fragmid

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   ...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

9. Hot Plasmids and Viral Preps - January 2021

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   ...AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8, as well as AAV5...

10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical...

11. Engaging with science and society at pgEd

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    ... in genetics research, their applications and regulatory policies; keeping tabs on the conversation about...

12. Overcoming the Challenges of Lentiviral Production

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    ...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...

13. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...Destination vectors. These vectors can induce combinatorial constitutive or inducible gene overexpression...

14. Adenoviral Vector Production and Troubleshooting

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    ...medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes, AdVs cannot...

15. Viral Vectors 101: Chemogenetics

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    ...PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed with the goal of creating...

16. Viral Vectors 101: Virus Safety

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    ..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-...

17. CRISPR in the Clinic

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    ... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...

18. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells. ABE8 efficiently edited the fetal...

19. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...

20. 5 Tips for Troubleshooting Viral Transductions

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    ...sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells), and has been shown to increase...