We narrowed to 643 results for: car

...differentiation between blue and white colonies. Take care in making your plates: X-gal is light and temperature...

...laboratory animals during in vivo research and the CARE guidelines for case reports are two sets of guidelines...

... die unless they have incorporated the plasmid carrying the required gene.  The table below lists some...

...Sergi, Jacob J. Hughey, Bryce T. Bajar, Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements...

...different plasmid backbones that varied in size or carried different genes. In these cases, gene expression...

...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

...of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment...

...incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna, Joanne Kamens, Lenny Teytelman, April...

...proteins will refold once denatured, however, so take care when using this method.      Fig 2:  A, ...

... be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for integration at a...

...Epitope availability References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

...proteins of interest - the secondary antibody needs careful consideration before selection. If all the primary...

...necessary result of the #MeToo movement. We are carefully attentive to genuine inclusion, but not worried...

...mushy, gooey contents of said containers, think carefully about how you want your software to treat those...

...distribute through our deposit process, and then we carefully track samples through our lab using barcoded tubes...

.... PubMed Central PMCID: PMC4139937. 3. Anders, Carolin, et al. "Structural basis of PAM-dependent target...

...like papers that can end with some sort of model (cartoons are appreciated) to visually sum up all of the...

... 4 °C prior to use. Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. Considerations...DNA tube. Incubate the mixture 15–20 min at RT. Carefully transfer the transfection mix to your HEK293T ...cells. Add the transfection mix dropwise, being careful not to dislodge the cells. Incubate the cells for...the following morning. The following morning, carefully aspirate the media. Replace the media with 15 ...

...move your microscope around the lab, be sure to carry it with two hands - one hand supporting the base...base and the other holding the arm - or use a cart. Revolve the nosepiece so that the lowest power objective...objective. Take your eyes away from the eyepiece and carefully revolve the nosepiece so that the next objective...will notice that the objectives get larger. Be careful to avoid hitting your sample with the objective...laboratory instrument, you should be sure to take care of your microscope. Bumps, scratches, and dust can...