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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 716 results for: cas

Showing: 501 - 520 of 716 results

  1. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...activation sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless... system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J....

  3. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to reproducibility...restrictions based on permissions, however, in some cases it would be possible to grant those wanting to understand...

  4. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...copy number is high (del Solar et al., 1998).  In cases of compatible plasmids, different negative regulation...replication or partitioning machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually...

  5. How to Be an Excellent Trainee

    Type
    Blog Post
    ...the first time. They can then plan accordingly in case you have questions or need a little assistance at...questions later – maybe even the next day. In the latter case, you can say “I usually need time to gather my thoughts...

  6. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...Addgene relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert...are highly repetitive, such as those containing cassettes of the same gene multiple times.  Bioinformatics...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...relies on dimerization for activation, only in this case ligand binding would bring the relevant ends together...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    ...initially isolated. Regrettably this is not usually the case if you obtain a cell line from a colleague. Time... been directly isolated from patients. In these cases, a test known as Short Tandem Repeat (STR) profiling...

  9. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...hydrogen bonds holding the DNA together, it recruits helicases to continue unwinding the strands. This creates...present in the chromosomal DNA ensure that the helicases are released from the DNA once the replication...

  10. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...research being published on our own topic. Only on occasion do we then have time to read about research from...video abstracts in science journals: An overview and case study." Journal of Librarianship and Scholarly Communication...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...density within a reasonable time-frame. For these cases, some strains carry an additional measure of control... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...is that my suggestions can help you make the best case for your candidacy. A major reason that a lot of...encouraged me to publish this manual and earn some cash for my expensive habits of running shoes and single...

  16. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...any additional work you might need to do. In most cases, I would recommend the second option for the experiment... gave me time to listen to some of my favorite podcasts. JUST GET STARTED - One Harvard Business Review...

  17. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... film or with specific imaging devices. In most cases, the primary antibody, which binds the protein of...inconsistent levels of the loading control. In these cases, the western should be repeated.  Finally, while...

  18. A Control for All Seasons

    Type
    Blog Post
    ... will increase the power of the results. In this case, mouse samples from positive (+), negative (-) and...antibodies will be highly specific, but in some cases they may cross-react and bind to more than their...

  19. Antibodies 101: Validation

    Type
    Blog Post
    ...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...

  20. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...designations all refer to the same test. On some occasions, there is an experimental group being compared... being between control vs. experimental. In this case, save yourself from family-wise error and just perform...
Showing: 501 - 520 of 716 results