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We narrowed to 986 results for: des

Showing: 501 - 520 of 986 results

  1. Addgene's Website Update

    Type
    Blog Post
    ...and the Addgene Help Center. A big part of this redesign was pulling all of our science options into a ...Addgene’s website easier to navigate, thanks to design choices informed by Web Content Accessibility Guidelines...word for it — check out all the new features and designs addgene.org! And when you’re finished, feel free...

  2. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...2022, from the launch of a new material to the redesign of a community resource. Now that we’re in the...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ...website saw 2.2 million visitors from 231 countries. Guides and protocols were our most popular content, while...

  3. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...Feng Zhang’s lab at the Broad Institute and was designed and cloned by Neville Sanjana. This fantastic ...your gRNA of interest to mammalian cells. It was designed to have increased lentiviral titer as compared...sampling of the research it has already been used for includes work probing mTOR signaling in cancer cells, deciphering...

  4. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...one-day conference aimed at the general public that provides an accessible introduction to various scientific...science cafes, and Reddit AMAs. Their website also provides a variety of short- and long-form written content... information about SITN: DayCon event website (includes archived content) Science in the News website ...

  5. Reflections and Looking Ahead

    Type
    Blog Post
    ...part of the story. This year, we made significant strides in making science more accessible and reliable....supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub...detailing antibody experimental results. We also redesigned our website to make it easier to find what you...

  6. Science as Art

    Type
    Blog Post
    ...of familiar flowers." This new x-ray technique provides scientist with a new method to explore the inner...of delicate plants and other organisms without destroying the original, which could provide a new tool ... collected by Darwin on his voyage. Previous destructive techniques were incompatible with the need to...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...it relates to other teams at the company. This provides new (and old) employees with information on how...

  8. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...spatial biology enthusiasts. This dataset currently includes 1049 community-validated reagents from 20 contributors... And the dataset is rich. Experimental data includes reagent type, target species, target name, host... inspired by the open-software community when designing the IBEX Knowledge-Base, seeking to create an ...

  9. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...happening inside cells for at least a couple of decades. Green is the classic color, but fluorescent proteins... greater than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent...illumination with far-red light. As the researchers described in Nature Communications last year, “The capability...

  10. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...et al., 2018). These sensors also exhibit high magnitudes of fluorescent responses. Studies on the kinetics...Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396...

  11. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...in the initiation of a vast number of signaling cascades, inducing different cellular responses. One of...localization of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate...protein signal transducers. The TRUPATH collection provides scientists with a toolkit to gain an understanding...

  12. An Addgene Summer Internship

    Type
    Blog Post
    ...instrument. They are the oldest sibling in a Guatemalan family of Mayan descent.   ...teaching me the basics of physics and sustainable design while allowing me to do my internship in the daytime...

  13. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...ve just discovered that your favorite gene was described in a CRISPR screen publication. You see this mentioned...This format is inherently flexible and allows the broadest possible scope of screens to be curated, for example...other than cell viability. The following video provides an in-depth walk through of how to use ORCS:   ...

  14. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    .... MBP has a similar function as GST in that it provides a method to express a foreign protein in bacteria...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...biotinylation and purification strategy outlined here provides a basis to determine binding kinetics using a ...

  15. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...time working through the details of experimental design and data with authors and referees. And I am constantly...visualization of science? Look at our graphics and design editors. How about condensing a week’s worth of...

  16. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ... to improve viral vector production. Whether redesigning viral packaging plasmids, investigating different...untreated and sucrose treated viral preparations.    Despite observing a positive effect on our overall yield...due to impaired endocytosis. In parallel assays designed to test the endocytosis of a fluorescent dye and...

  17. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials,...California Davis School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging...supporting hybridoma sharing within the community for decades. Educational Resources The Basics of Western Blotting...

  18. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...conditions. In those cases, pay special attention to designing your controls in validation, as you may need to...help you avoid a great deal of frustration when designing and troubleshooting immunoimaging assays. It can...

  19. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...blocks of polymers are heated up and pulled to a desired thickness. The fibers are drawn with microelectronics...whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic channels. These...using NeuroStack and the fibers as previously described, then measured electrophysiological potential ...

  20. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...packaged, when the vector is expressed. Once the design and construction steps are complete, it’s time ...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...
Showing: 501 - 520 of 986 results