We narrowed to 625 results for: emb

1. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

4. Hot Plasmids - February 2022

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   Blog Post

   ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

5. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

6. Antibodies 101: Isotypes

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   ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

7. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

8. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

9. Multiplex Genome Editing with CRISPR-Cpf1

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   ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

10. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

11. Fluorescent Proteins 101: Luciferases

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    Blog Post

    ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

12. Viral Vectors 101: Viral Applications

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    Blog Post

    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

13. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

14. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

16. Summer SciComm: Preprints

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    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

17. Antibodies 101: Affinity Reagents

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    Blog Post

    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

18. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

19. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

    ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

20. Choosing the B(right)est Fluorescent Protein: Photostability

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    Blog Post

    ...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...