We narrowed to 630 results for: emb

1. Resume Writing for Non-academic Science Careers

   Type

   Blog Post

   ...skills, and the job you are applying for. Also remember that a resume is typically no more than 2 pages...

2. Advice for Scientists Starting a Lab

   Type

   Blog Post

   ...scientists to their labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early ...

3. Hot Plasmids - February 2022

   Type

   Blog Post

   ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

4. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

5. Antibodies 101: Isotypes

   Type

   Blog Post

   ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

7. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

8. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

9. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   ...ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes be removed ...

10. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

11. Fluorescent Proteins 101: Luciferases

    Type

    Blog Post

    ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

12. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

13. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

14. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

15. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

16. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

18. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

19. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

20. Summer SciComm: Preprints

    Type

    Blog Post

    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...