We narrowed to 637 results for: erf

1. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

   ...sequence. The RNA-targeting ability of Cas13 makes it perfect for targeting mRNA and useful as a mechanism of...

2. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

   ...the very nature of the way the experiments were performed, they did not see anything unique to male stem...

3. Plasmids 101: Golden Gate Cloning

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   Blog Post

   ...exciting applications, this system is an incredibly powerful tool for cloning complicated constructs in a single...

4. Plasmids 101: Cre-lox

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   Blog Post

   ...the selection marker, Cre can be used to easily perform this eviction. Regulated Cre expression – placing...

5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...the experiment. Handling can however negatively interfere with certain tests and is not always recommended...

6. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...your parental mice to generate F1 Once you’ve performed your first cross, congratulations! You’ve generated...

7. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

   ...researchers with useful and adaptable tools to perform basic and preclinical research. For scientists ...

8. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

   ...soCoChR podcast segment Optogenetic stimulation is a powerful tool used by neuroscientists to interrogate neuronal...

9. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   Blog Post

   ... is part of a particular protein complex. Not performed in a native cell context. For AP-MS, cells are...

10. Lab Automation at Addgene

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    Blog Post

    ...Platform and it suits our plasmid prepping needs perfectly. Working with Qiagen, we developed a protocol ...

11. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...loadingExample: AcrIIC2 interactions with Cas9 interferes and prevents the correct assembly of the crRNA-Cas...

12. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...98.3%, or 98.6% efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold. The ability of...

13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post

    ...demethylation – tight binding is crucial for dCas9 to interfere with DNMT1 even through replication. The dCas9...

14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

    ...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ... study the mechanisms of infection and how to interfere with it in a BSL-2 lab instead of a BSL-3 lab....

16. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post

    ...talk – to win the audience’s applause with your performance. When your institute opens its doors to the public...

17. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...methods flood the cell with protein, which can both interfere with a cell’s normal function and result in a ...

18. Viral Vectors 101: Calcium Sensors

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    Blog Post

    ...measurements are more susceptible to artifacts and pH interference, and comparison between experiments can be more...

19. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post

    ...SplitSet™. With these tools, NEB successfully performed a 35-fragment Golden Gate reaction, using four-base...

20. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...Regardless of which ligand you choose, there is no “perfect” drug that only binds to one receptor, so it is...