We narrowed to 639 results for: erf

1. Plasmids 101: Golden Gate Cloning

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   ...exciting applications, this system is an incredibly powerful tool for cloning complicated constructs in a single...

2. Plasmids 101: Cre-lox

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   ...the selection marker, Cre can be used to easily perform this eviction. Regulated Cre expression – placing...

3. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...the experiment. Handling can however negatively interfere with certain tests and is not always recommended...

4. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...your parental mice to generate F1 Once you’ve performed your first cross, congratulations! You’ve generated...

5. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...researchers with useful and adaptable tools to perform basic and preclinical research. For scientists ...

6. Viral Vectors 101: Calcium Sensors

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   ...measurements are more susceptible to artifacts and pH interference, and comparison between experiments can be more...

7. High Complexity Golden Gate Assembly with NEB’s DAD

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   ...SplitSet™. With these tools, NEB successfully performed a 35-fragment Golden Gate reaction, using four-base...

8. Viral Vectors 101: Chemogenetics

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   ...Regardless of which ligand you choose, there is no “perfect” drug that only binds to one receptor, so it is...

9. Antibodies 101: Choosing the Right Isotype

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   ...antigen in a shorter period of time and would likely perform better for probing poorly expressed targets. Similarly...

10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...loadingExample: AcrIIC2 interactions with Cas9 interferes and prevents the correct assembly of the crRNA-Cas...

11. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...demethylation – tight binding is crucial for dCas9 to interfere with DNMT1 even through replication. The dCas9...

12. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

13. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ... study the mechanisms of infection and how to interfere with it in a BSL-2 lab instead of a BSL-3 lab....

14. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...98.3%, or 98.6% efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold. The ability of...

15. Lab Automation at Addgene

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    ...Platform and it suits our plasmid prepping needs perfectly. Working with Qiagen, we developed a protocol ...

16. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...population. These pre-existing antibodies can interfere with virus-cell interactions and seriously hinder...

17. Progress Towards a PAM-Free CRISPR

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    ...sequence that you hope is present next to the "perfect" guide sequence for your genome engineering experiment...

18. A Look at Addgene's QC Process

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    ...Nobody’s perfect! This is a platitude I’m sure we have all heard. It’s a truth that extends to both people...

19. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...soCoChR podcast segment Optogenetic stimulation is a powerful tool used by neuroscientists to interrogate neuronal...

20. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ... is part of a particular protein complex. Not performed in a native cell context. For AP-MS, cells are...