We narrowed to 709 results for: esc

1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   Blog Post

   ... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy...

2. Adenoviral Vector Production and Troubleshooting

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   Blog Post

   ..., can accommodate large transgenes, transduce quiescent and dividing cells, and do not integrate into ...

3. Rabies and Neuronal Tracing

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   Blog Post

   ...widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain...

4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   Blog Post

   ...ultrasonic frequencies (> 20 kHz, produced from fluorescent light bulbs, for example) that are outside the...

5. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   ...Addgene In addition to the wealth of open resources described above, there were many plasmid tools presented...

6. Corralling and Cornering the Goldilocks Antibody

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   Blog Post

   ...secondary antibodies, allowing us to only image autofluorescence i.e., background noise. We then compare the...

7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...expressing these constructs can be achieved by fluorescence-activated cell sorting, and stable gRNA expression...

8. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   Blog Post

   ...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...

9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   ...the repertoire of antibodies from 19 COVID-19 convalescent individuals (Tong et al., 2021). Another version...

10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ... use a modified version of the Python program described by Joung et al. 2017, which we hope to make available...

11. A Look at Addgene's QC Process

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    Blog Post

    ...types of data for each of their plasmids, from descriptions of key components of the plasmids to partial...

12. Design Tips for Prime Editing

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    Blog Post

    ...but this also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation...

13. Making CRISPR Plasmids Using Fragmid

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    Blog Post

    ...Wen, B., Kraus, C., Miller, N. W., Steger, R. J., Escude Velasco, B., Bosch, J. A., Zirin, J. D., Viswanatha...

14. Progress Towards a PAM-Free CRISPR

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    Blog Post

    ...Between SpRY and several of the other Cas variants described, nearly all genomic landscapes should be editable...

15. Tips for Technical Support Calls

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    Blog Post

    ...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...

16. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    Blog Post

    ...driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research from its systemic flaws...

17. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post

    ...ability to complete the project on such a short timescale. Our article ultimately reported the development...

18. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of...

19. Tips for Titering Your Lentiviral Preps

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    Blog Post

    ...is particularly useful when vectors carrying fluorescent markers are used as it eliminates the need for...

20. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    Blog Post

    ...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...