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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 850 results for: met

Showing: 501 - 520 of 850 results

  1. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...respond to friends asking me to dinner saying something like, “I’ll be there in a few minutes, I just ...mind for the endpoints of a task or project (with metrics if possible) and, at the outset of a task, I outline...visit to a university. My to-do list might look something like the image on the right (and for those of ...

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...presentation or Ted Talk. You want to give the audience something to think about, argue with, or learn from. Some...comes. 5. Choose interesting content Talk about something you know a lot about and that you care about. ...with your confidence. It is best not to be asked something that you don't know the answer to, but if you ...

  3. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ... the right lab such a big deal? It’s actually something you CAN choose and it will make a huge difference... help identify your priorities and needs, but sometimes it just takes introspective thought on what’s ...such as the Future of Research Symposium and #MeTooSTEM, academic bullies are hard to stop—they often ...

  4. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...specificity High sensitivity Easy expression Gives ratiometric data The result was a fusion protein called ...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting..., A. C. H., Gokal, D., Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song, X., Wang, G., Su, X.-...

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... strong early gene expression, but can become methylated over time, resulting in decreased expression ...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic...hydroxylase promoter in vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org...

  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other...multiplexed over 65 targets!) IF is a versatile method with endless possibilities, and there are always...Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org/10.13070/mm.en.9.2846 Radtke...

  8. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...Crystal Violet Pre and Post Visible Light 100 Methylene Blue Post Visible Light 100 Ethidium Bromide...from the gel via your favorite gel purification method, it is important to determine the concentration...calculate this based on DNA concentration alone. One method is to conduct 2 ligations for each plasmid you ...

  9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...and volumes to determine the optimal injection parameters needed to transduce your cells of interest. Using...Once you have determined the optimal injection parameters for the serotype that best transduces your cells...performance in your system. It is very rare for a methods section to include all of the variables that need...

  11. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826...genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903. PubMed...

  12. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...studying its structure can help us understand the parameters of CRISPR experimental design. Let’s dive into...is recognizing the PAM on DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide...al., 2013). As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after...

  13. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov tells us...modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically...

  14. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what impact looks...like to measure yours? More Reading Fenner M. "Altmetrics and Other Novel Measures for Scientific Impact...

  16. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...of listening to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science...

  19. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3 mRNA binding...

  20. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ... in terms of contamination, but consider flow cytometers. These fluidic machines handle biological samples...markets. Rethinking our approach to lab waste is something we all need to do. Remember, it was only 50 years...
Showing: 501 - 520 of 850 results