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We narrowed to 779 results for: mos

Showing: 501 - 520 of 779 results

  2. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...Nonsense! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism...

  3. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...signal and can be used in light-sensitive systems. Most excitingly, assembly of the split luciferase is ...

  4. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...field of research], and have excellent [list the most important skills for the position]. I am enthusiastic... does not need to be a literary masterpiece. For most jobs, the writing quality required will be close...

  5. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...

  6. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...several sgRNA’s at the same time, and choose the most effective ones to increase your chances of gene ...the pronuclei were fusing, you may have several mosaic mice. To figure out what alleles are present in...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...included two reference tables below listing some of the most common bacterial and mammalian promoters. These ...mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less active...

  8. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting as a full-fledged...Containing the spread of this virus has become an utmost priority for countries around the world and to ...

  9. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors...pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1...

  10. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...RNA genomes into DNA to be integrated into the chromosome of the cell. This integrated viral genome is ...than regular lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...scientists using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable...2014). While 10 derivatives of TO exist, Mango is most frequently used with TO1-biotin or TO3-biotin (the...

  12. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...premature aging, and other harmful processes. The most dangerous type of DNA lesion results from breakage...double-strand break repair and their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (...

  13. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...labeling cells for flow cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated...antibodies but on small molecule dyes, are useful, most notably when it comes to viability and proliferation...

  14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... will be represented through FSC-A vs. SSC-A, so most people use that setting. Figure 2: As the...the two cell types (likely B cells and others). Almost no cells stain positively for both markers. When...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength to use but can...publication, including the supplemental data. You’re most likely to find thorough characterization data, like...

  16. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...several ways to achieve multiplex IF. One of the most common approaches is to use sequential cycles of...are a few specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...research is needed to determine which recA- strains are most effective at reducing multimerization and define...of Multimeric Forms of Circular Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128...

  18. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas of the membrane... x-ray film or with specific imaging devices. In most cases, the primary antibody, which binds the protein...

  20. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing system derived... conventional methods. First, one can work with almost any strains of mice, as compared with conventional...
Showing: 501 - 520 of 779 results