We narrowed to 563 results for: pac

1. Hot Plasmids: Winter 2025

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   Blog Post

   ...from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). Figure...

2. Magnetic Control of Proteins: More than a Dream

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   ...-up studies. “We don’t have a vision of passive impact,” he says, noting that his team regularly shares...

3. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   ...that are distant linearly but close together in space. The chromatin “picture” is then developed by detecting...

4. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

6. Transferable Skills Guide: Managing a Team

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   ...and not valued which will undoubtedly negatively impact your work and your life. It’s up to you to decide...

7. Plasmids 101: Optimizing Plasmid Yields

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   ...strain Differences between E. coli host strains can impact plasmid yield. Check the bacterial strain to see...

8. Experimenting with New Careers while in Grad School

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   ... once a week to get recharged. The fun change of pace helped break up the negativity and allowed me to...

9. Genome-wide Screening Using CRISPR

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   ...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...

10. Which Fluorescent Protein Should I Use?

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    ...wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs are fluorescent when ectopically...

11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future of the...

12. Great Results Start with Great Standard Curves

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    ... be using ahead of time to see what factors may impact the dynamic range of the assay. Try to minimize...

13. DNA Purification Without a Kit

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    ...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...

14. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...gene function while using fewer sgRNAs. These new compact libraries will allow scientists to conduct genome-wide...

15. Transferable Skills Guide: Identifying Your Transferable Skills

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    ... is the transferable skills that create a full package and add an extra shine to your work. Knowing your...

16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ...affinity for endogenous molecular targets such as paclitaxel. Unlike other systems, no genetically encoded...

17. CRISPR Antimicrobials

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    ...containing a phage origin of replication that can be packaged into replication-incompetent phage particles using...

18. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...materials, protocols, and courses, as well as a space for research labs to create their own lab or group...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...decrease. Furthermore, the size of the parts also impacts ligation efficiency, as concentration does not ...

20. CRISPR 101: Any Base Transversion Editors

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    ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...