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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 846 results for: tat

Showing: 501 - 520 of 846 results

  1. MXS Chaining

    Type
    Blog Post
    ...Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular...genes from organisms with high CpG dinucleotide representation. The specific restriction enzymes used in various... 3. The goal of high throughput cloning is to facilitate the construction of plasmids with many components...

  2. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...acceptor FRET know-how with aptamers, aptabeacons (or aptatags) were born. Aptabeacons can be created in many...general premise is that an aptamer in its unbound state contains a fluorophore and a quencher in close proximity...brighter version of the aptamer, but it still had limitations in vivo. Using a combination of SELEX and fluorescence-activated...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ...editors, PE6a–g, that overcome some current prime limitations and enable impressive editing efficiencies in...similar or better efficiency than PEmax, the current state-of-the-art. PE6b was very efficient for short, unstructured...transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD...

  4. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...get there: I had to send the company detailed documentation of my results, answer loads of questions, and...differences in customers’ personalities and their expectations. If you are willing to listen to a customer’...support roles. Examples are Technical Service Representative, Technical Support Specialist, Technical Support...

  5. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    .... In vivo, these recombination reactions are facilitated by the recombination of attachment sites from...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry vector.... to 4 DNA fragments, in a specific order and orientation, in one tube, into one Gateway vector to produce...

  6. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  This process...For example, multiple studies show that certain mutations of the BAP1 gene play a role in susceptibility...individuals, asbestos has not been banned in the United States and many other countries, and it is still imported...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...at the same time increasing interdisciplinary citations in their own work. Of course not all research ...ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their contribution...Archambault. "The decline in the concentration of citations, 1900–2007." Journal of the American Society for...

  8. Molecular Biology Reference

    Type
    Guide
    ...elements often determines the type of plasmid and dictates how it might be used in the lab. Below are some...common plasmid types: Cloning Plasmids - Used to facilitate the cloning of DNA fragments. Cloning vectors... gene function as well as identify changes or mutations that may cause certain diseases. Sequencing DNA...

  9. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...maintaining their friendships. I put “neutral” in quotations here because I would not have considered myself...handy: Empathy: If you often find yourself in confrontational situations with others because you simply cannot...competing, or accommodating, if you find yourself gravitating most frequently towards these approaches, you...

  10. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...training and experience, better align trainee expectations with job outcomes, and increase diversity in...need to know in order to even assess the current state of research became more clear throughout the various...information publicly available may realign trainee expectations with actual job outcomes and/or disincentivize...

  11. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...research is dependent on a pathology microtome/cryostat, then making a careful decision on the condition...for suggestions to meet each others’ needs and limitations. Everything is negotiable. Try asking for discounts...research, then it’s worth working within their limitations. 3. Always provide value to others This is a ...

  12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...have intricacies that aren’t always mentioned in static written protocols. It is therefore a bit ridiculous...experiments with too few replicates leading to suspect statistical significance (you can read more about this problem...experimental design practices including talking to statisticians when necessary and encouraging interlab peer...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...across the genome) has been implicated in disease states, and targeting the epigenome may make certain processes... spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves methylation kinetics such that cytosine...transduction. Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9...

  14. Finding Your Perfect Job After University

    Type
    Blog Post
    ...of hands-on lab work for a dissertation. I chose to carry out my dissertation research in a lab in California...outcome for a specific combination of genetic mutations. This is a particular challenge in pancreatic ...

  15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...Kishor Jadhav. Mycoplasmas can remain in the dry state for a longer time. When they come in contact with... is required in this process because result interpretation might be difficult. The low density of mycoplasmas...by these bacteria can hinder correct result interpretation. Also, the signals from the nuclear area make...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Location, location, location! What do buying real estate and using AAVs have in common? The three most important... location, location! Just like this common real estate mantra, the choice of serotype (which determines...identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory connections (3). These facts beg the question...

  17. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ...work in certain applications — the most common limitation is that certain probes are not very good at crossing...

  18. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...biochemist, a geneticist, a microscopist, a biostatistician, and that one golden-handed technician who ...Andrew, the team’s founder, to create a mission statement in the first few meetings and we’ve volunteered...like kudzu. Think of a soccer player who gets a reputation for taking risky shots at the goal rather than...

  19. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... to assemble several DNA parts in a specific orientation using Type IIS restriction enzymes (Weber et ... researchers to expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified...acssynbio.3c00423. Pérez-González, Ana, et al. “Adaptation of the GoldenBraid Modular Cloning System and...

  20. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...previously-designed tyr gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic...recombineering, with 96-99% efficiency for point mutations and 66% efficiency for small insertions. Multiplexing...degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are delivered on a different...
Showing: 501 - 520 of 846 results