We narrowed to 712 results for: BASE
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TypeBlog Post...expression. AAV retrograde Unlike traditional dye-based neuronal tracing reagents, AAV particles require...
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Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...consistent with the cell type that was predicted based on the Atlas. They also looked at 17 of these TFs... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...activation of transcription in bacteria by co-opting σ54-based transcription. Transcription from σ54 promoters ... -
Components of CRISPR/Cas9
TypeBlog Post... sequence contained within the guide RNA and a 3-base pair sequence known as the Protospacer Adjacent ... -
Finding and Joining Your Dream Lab
TypeBlog Post...interests.” George Church selects new hires initially based on those who notice and embrace the “odd and extreme... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...liability in respect to actions taken or not taken based on any or all the contents of this post. An attorney...September 2017, USCIS received 116,224 employment based green card applications and approved 91,023 during... -
Antibody Validation for Flow Cytometry
TypeBlog Post... Knockout cell line approach Genetic knockout-based validation can conclusively prove the ability of... through its ability to separate the cell lines based on expression of the target. In this case, you could... -
Retrograde AAV viral preps
TypeCollection...Available Retrograde AAV See our retrograde AAV based on the functional catagories listed below. Narrow... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...NIR photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...weeks of exposure to pH 5.1 (Potter et al., 2014). Based on our results and the results reported elsewhere... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...specificity. This can be especially important for base editing techniques, where Cas9 must be directed ... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune cells... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post..., SRIRACCHA and GEMCherry2. We will cover single base-pair editing CRISPR reporters in the near future... -
Hot Plasmids - May 2022
TypeBlog Post... Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang... system has a much lower error rate. For a one kilobase insertion, the researchers reported an editing...AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...that the antibody is guaranteed to work. Antibody-based assays are complex and antibodies may perform differently... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...is one of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101... -
Antibodies 101: Secondary Antibodies
TypeBlog Post... to identify each protein of interest, this gel-based method identifies proteins solely by weight and ... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene...