... sequence contained within the guide RNA and a 3-base pair sequence known as the Protospacer Adjacent ...

...interests.” George Church selects new hires initially based on those who notice and embrace the “odd and extreme...

...liability in respect to actions taken or not taken based on any or all the contents of this post.  An attorney...September 2017, USCIS received 116,224 employment based green card applications and approved 91,023 during...

... Knockout cell line approach Genetic knockout-based validation can conclusively prove the ability of... through its ability to separate the cell lines based on expression of the target. In this case, you could...

..., SRIRACCHA and GEMCherry2. We will cover single base-pair editing CRISPR reporters in the near future...

...specificity. This can be especially important for base editing techniques, where Cas9 must be directed ...

...NIR photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while...

...weeks of exposure to pH 5.1 (Potter et al., 2014). Based on our results and the results reported elsewhere...

...Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-...

... Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang... system has a much lower error rate. For a one kilobase insertion, the researchers reported an editing...AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky...

...that the antibody is guaranteed to work. Antibody-based assays are complex and antibodies may perform differently...

...is one of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101...

... to identify each protein of interest, this gel-based method identifies proteins solely by weight and ...

...about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene...

...statement. Make certain to choose your recommenders based on your relationships to the person as well as their...resources, we created STEAMid, for a nationwide database of  STEAM internships for high school, college...

...screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune cells...

...wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed...

...Delivery: Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine...

...gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10...

.... Such disparities might suggest underlying sex-based influences on physiologic or pathologic processes...