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We narrowed to 717 results for: CaS

Showing: 521 - 540 of 717 results

  1. Antibodies 101: Validation

    Type
    Blog Post
    ...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...

  3. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...antibodies together in the same color panel. In this case, make sure to compare the excitation/emission spectra... so it's better to use a brighter fluorophore in case the CD45 expression turns out to be weak. Table ...

  4. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...been infected with a microbe, to trigger immune cascades that clear these infections. Anything that generates...immune response and initiates different immune cascades. In the research setting, antibodies of different...

  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP...

  6. Deep Dive: qPCR

    Type
    Blog Post
    ... double peak even with a single product. In that case, you’ll want to validate your primer/probe through...Higgins, 2000; Ref, Zhu, 2002), though there are cases in which it is an appropriate choice (Panina, 2018...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...ability to cross the BBB, or the PNS, while in many cases targeting the liver less. Because these systemic...being geographically constrained to the brain. In cases where it’s critical to minimize or eliminate off-target...

  10. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

  11. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ... were absolutely abuzz with anticipation as they cast their ballots for their favorite submission. So ...

  12. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...it's hard to summarize, but we wanted to mark the occasion by trying.  When we started in 2004, we were...

  16. Call for Guest Bloggers

    Type
    Blog Post
    ...editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein...

  19. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...Halloween and how seriously we take costume design. In case you need more convincing, I’ve got just the proof...
Showing: 521 - 540 of 717 results