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We narrowed to 993 results for: Des

Showing: 521 - 540 of 993 results

  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs... at 3 days post fertilization. Since Ma et al. designed a repair template with two synonymous mutations...thus quieting some fears about the creation of “designer babies.” The differences in editing between iPS...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...ortholog requires a different PAM sequence, a gRNA designed for one dCas9 should be specific to that ortholog...boxB Magenta boxB-MS2 White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently...genome editing. Such a system has been previously described for transcriptional activation/repression and ...

  3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...GFP-labeled cells. Once Tang and Cepko found a description of GFP-binding nanobodies, the project really...stable inside cells. Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies...green-red Cre-lox systems. T-DDOGs can also be designed with other DNA-binding domains, including the ...

  4. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...growing dialogue on the state of a system that, despite all of its successes, has many deep-rooted flaws...all bad, however - there are some signs that the tides may be turning. In 2013, the NIH announced its first...in Scientific Training (BEST) awards, which are designed to support new approaches that address the need...

  5. Brain Armamentarium

    Type
    Collection
    ...below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 230681...pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA AiE2116m is an enhancer sequence, designed to drive AAV-mediated transgene expression in ...

  6. Optogenetics AAV Preps

    Type
    Collection
    ... (soma-targeted) mRuby2 Cre dependent 9 Hillel Adesnik 124603 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40... (soma-targeted) mRuby3 Cre dependent 9 Hillel Adesnik 124651 pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 CaMKII... (soma-targeted) mRuby3 Cre dependent 9 Hillel Adesnik 137155 pAAV-nEF-Con/Fon-iC++-EYFP nEF iC++ EYFP...

  7. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...non-coding RNAs (lncRNA). lncRNAs are over 200 nucleotides in length and lack an open reading frame, so ...transcription machinery of a target gene or DNA that codes for ncRNA. CRISPRa, on the other hand, recruits ...which targets RNA instead of DNA and selectively degrades RNA molecules. This system is often used to knock...

  8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...throughout the library preparation process and includes Picogreen quantification of various intermediate...sequences for Illumina sequencing as well as unique barcodes per sample so we can assign sequencing reads to... use a modified version of the Python program described by Joung et al. 2017, which we hope to make available...

  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... There are several calcium reporters specially designed for the aims and requirements of in vivo studies...populations of interest. Many of the calcium sensors described are also already packaged into viral vectors and... L, Campbell RE. Structure-and mechanism-guided design of single fluorescent protein-based biosensors....

  10. Tips for Technical Support Calls

    Type
    Blog Post
    ...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...scientists answering their technical support questions (besides Addgene, I know both Abcam and NEB have Ph.D.s ...believe that scientists who contact customer service deserve prompt, courteous responses. No one is likely to...

  11. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ... try amplifying the viral vector with bacteria designed to minimize rearrangements such as NEB Stable ...production of virus. Alternatively, if the virus encodes a fluorescent protein, the packaging cells could...working with, there’s a lot that goes into virus design, production, and transduction. Once you’ve harvested...

  12. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... This vastly increases the possibilities when designing screens. Each serotype has its own tropism, or...experiment. Additional considerations One of the downsides to using AAV is the limited packaging capacity...Adames, A. C., Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature...

  13. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...positive and negative populations, Careful panel design In both conventional and spectral flow cytometry...indistinguishable. This tool is of particular value when designing a panel.     Figure 3: Similarity index...if markers are co-expressed in your cells when designing your panel. If you have two highly overlapping...

  15. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ... also the easiest: don't use a DNA vector that encodes both Cas9 and sgRNA. What if I want to work with...one mistake can be enough. Reversal drives are designed to overwrite a previous gene drive and thereby... on popular support, which in turn depends on widespread trust in scientists to develop new technologies...

  16. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...terrific getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs...matrix BLOSUM62, but you may want to check the description of the other matrices to see if another would...alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ...

  17. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... the data points generated by replicate values provides useful information about the accuracy of the curve...If the unknown sample’s measurement is too low, redesign the standard curve dilution series with lower ...often recommend using advanced graphing software designed to plot complex trendlines. If you don’t have ...

  18. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... can teach  5-10 year olds in a single evening. Grades K-5 cover a broad range of attention spans, skill...CRISPR work? This section was essentially background slides getting everyone on the same page in terms of the... by running it by the teachers and sharing some slides on Twitter, where I was able to get some great ...

  19. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly used for ...DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance by expressing a protein that...experiments in which off-target mutations are undesirable. Pros: Works across many species/systems. Cons...

  20. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Microscopy. What is Expansion Microscopy?  ExM provides a straightforward answer to the space problem....improve label density (Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially...Bournonville L, et al (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria ...
Showing: 521 - 540 of 993 results