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.... MBP has a similar function as GST in that it provides a method to express a foreign protein in bacteria...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...biotinylation and purification strategy outlined here provides a basis to determine binding kinetics using a ...

...blocks of polymers are heated up and pulled to a desired thickness. The fibers are drawn with microelectronics...whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic channels. These...using NeuroStack and the fibers as previously described, then measured electrophysiological potential ...

...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials,...California Davis School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging...supporting hybridoma sharing within the community for decades. Educational Resources The Basics of Western Blotting...

...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...conditions. In those cases, pay special attention to designing your controls in validation, as you may need to...help you avoid a great deal of frustration when designing and troubleshooting immunoimaging assays. It can...

...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...packaged, when the vector is expressed. Once the design and construction steps are complete, it’s time ...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...

...inversions, deletions, and translocations. It also provides researchers with many different ways to control...the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation process...experiment? In this post, Addgenie Chari Cortez guides us through some common controls and why you might...

... the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C....America. To use this SEC-based system, first design and clone 500-700 bp homology arms for your gene...injected 60-80 animals per gene, they obtained the desired insertions on the first attempt for 6/7 genes. ...

...As with other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological atypicalities... Joanne Kamens, Executive Director  I Contain Multitudes by Ed Yong "Microbes are everywhere and this ...shocking at times."     - Jason Snair, Product Designer Sarum: The Novel of England by Edward Rutherfurd...

...transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique isoforms... and screening mechanisms, although the unique barcodes for each TF help streamline identification. While...a disease in a model system. TF screens can be designed to identify custom TF programs to acutely initiate...

...distribution of cell lines from repositories on opposite sides of the world shows that science is truly a global... et al., 2006), the hACE2 transgenic mice were designed after it was discovered that SARS-CoV, which entered...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the ...

...abstracts were driven by the quality of the study design or by their preconceived ideas about the controversial...transmission and how different fabrics and mask designs could work to slow the spread of illness. I later...Series: Masters of SciCommSummer Scicomm Series: Modes of CommunicationCareers in Science Communication...

...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...bacteria. In a gram negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan... is also useful in a laboratory setting. When designing a plasmid to be inserted into a specific bacterium...

...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

...imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein...brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will work better with shorter...

...engineering approaches and scientific know-how to design/build/test their own project ideas using synthetic...newsletters, pairs teams with STEM mentors, and provides a nationally recognized platform in March to exhibit...s BioBuilderClub teams’ biological engineering designs and ideas ranged from a biological breathalyzer...

...positive effect on cancer treatment (Ref 1). As described in the presentation, starvation or the use of ...field of Immuno-Oncology, a sizzling hot topic, includes studies on novel cancer therapeutics such as  ... with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel...

...tables with fluorescent protein properties and provides a quick way to compare fluorescent proteins, e.g...been used in yeast by Lee et al (2013) and in nematodes by El Mouridi et al (2017) and Heppert et al (...practical brightness. Therefore, practical brightness provides a better picture of what can be expected in a ...

...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...sequence NGG TTTV PAM site preserved? Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing....  Similar to a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in ...

... craft sentences for abstracts and presentation slides, learn from audiences as they do science outreach...about whether the same principle applies to other slides in your deck.  If someone asks a question about...of a new science communication initiative that provides yet more scientists with opportunities to practice...

...immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works...up the DNA, or enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires...both tissue or cell type, amount of protein, and desired fragment length for your output assay. A longer...