We narrowed to 639 results for: ESP

1. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...interest may be very limited with this method, especially in adult animals, and not targeted to a specific...

2. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...reproduction include embryonic lethality, a short lifespan, or infertility and can arise due to copy number...

3. Hot Plasmids and Viral Preps - March 2021

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   ...The AID system relies on a transport inhibitor response 1 (TIR1) expressed under a desired promoter with...

4. Plasmids 101: Modular Cloning

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   ... markers, promoters, enhancers, etc. MoClo is especially useful for more complex CRISPR experiments (e.g...

5. Hot Plasmids - October 2020

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   ...to label excitatory and inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind...

6. How to Prepare for an Industry Interview

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   ...that have lots of general questions about job responsibilities, performance evaluations, professional development...

7. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...atmosphere, and the people.” My absolute favorite response to this question came from Lucy Hammer, our Financial...

8. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ...makes wide use of retroviral delivery systems, especially lentiviral vector-based CRISPR pooled libraries...

9. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ... along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality. Since...

10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ... not all, S. pombe Met genes complement the corresponding S. cerevisiae met deficiencies (manuscript in...

11. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...efficient oligomerization of target proteins in response to blue light. This tool, CRY2clust, was created...

12. Lab Automation at Addgene

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    ...helpful machines I have used in the lab. It’s especially useful if you want to avoid sore fingers. There...

13. Fluorescent Proteins 101: Introduction to FRET

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    ... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...

14. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...represents reality as accurately as possible. This is especially true in the case of protein tagging to elucidate...

15. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...organisms , they have many levels of complexity, especially when trying to explore the links between genetics...

16. Degrading DNA with Cascade-Cas3

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    ...The nuclease domain of Cas3 is considered weak, especially compared to the power of Cas9. However, by continually...

17. Common Injection Routes in Mice

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    ...intradermal injections is studying inflammatory responses, as this method is useful for analyzing localized...

18. Viral Vectors 101: Producing Your rAAV

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    ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

19. Plasmids 101: Degron Tags

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    ...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...

20. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...fluorescent molecules conjugated together) can be especially sensitive to batch effect. For example, when ...