We narrowed to 637 results for: ESP

1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

   ...efficient oligomerization of target proteins in response to blue light. This tool, CRY2clust, was created...

2. Fluorescent Proteins 101: Introduction to FRET

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   Blog Post

   ... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...

3. Lab Automation at Addgene

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   Blog Post

   ...helpful machines I have used in the lab. It’s especially useful if you want to avoid sore fingers. There...

4. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   ...represents reality as accurately as possible. This is especially true in the case of protein tagging to elucidate...

5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   Blog Post

   ...interest may be very limited with this method, especially in adult animals, and not targeted to a specific...

6. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   Blog Post

   ...reproduction include embryonic lethality, a short lifespan, or infertility and can arise due to copy number...

7. Hot Plasmids - October 2020

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   Blog Post

   ...to label excitatory and inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind...

8. Plasmids 101: Modular Cloning

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   ... markers, promoters, enhancers, etc. MoClo is especially useful for more complex CRISPR experiments (e.g...

9. A Look at Addgene's QC Process

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   Blog Post

   ... part of maintaining the quality of reagents, especially shared ones.  The importance of QC Plasmids ...

10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

    ... poses the risk of disrupting adjacent genes, especially those regulated by or overlapping with the lncRNA...

11. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post

    ...are a gold mine of parts for synthetic biology, especially when combined with natural inducible expression...

12. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...The AID system relies on a transport inhibitor response 1 (TIR1) expressed under a desired promoter with...

13. How to Prepare for an Industry Interview

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    Blog Post

    ...that have lots of general questions about job responsibilities, performance evaluations, professional development...

14. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    Blog Post

    ...atmosphere, and the people.” My absolute favorite response to this question came from Lucy Hammer, our Financial...

15. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...organisms , they have many levels of complexity, especially when trying to explore the links between genetics...

16. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...fibroblasts via retroviral and lentiviral infections respectively [9, 10]. These stellar works led to a Nobel ...

17. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

    ...CRISPR protein tagging does have its limitations, especially when trying to perform high-throughput experiments...

18. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    ... then handled. Additionally, many cell lines, especially the endocrine cell lines we most commonly work...

19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post

    ...commonly FPs with red and green wavelengths, respectively, are selected (6). These previous blog posts...

20. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post

    ...monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed mK-GO somewhat...